ASTM D5246-15 PDF

SIGNIFICANCE AND USE 5.1 Pseudomonas aeruginosa is an opportunistic pathogen, and has been linked as the causative agent of numerous infections that may be transmitted through a contaminated water supply to a susceptible host. Note 1: Fecal waste is >95 % E. coli which is found in humans and warm bloodied animals. 5.2 The membrane filtration procedure described is a rapid and reliable test method of detecting P. aeruginosa in water. SCOPE 1.1 The test method covers the isolation and enumeration of Pseudomonas aeruginosa. Testing was performed on spiked samples using reagent grade water as the diluent from surface waters; recreational waters; ground water, water supplies; especially rural nonchlorinated sources; waste water; and saline waters. The detection limit of this test method is one microorganism per 100 mL. 1.2 This test method was used successfully with reagent water. It is the user's responsibility to ensure the validity of this test method for surface waters, recreational waters, ground water, rural nonchlorinated sources; waste water; and saline waters. 1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 10.