BS EN ISO 6579-4:2025

1   Scope This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium ( 1 ,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. The method is applicable for:

— differentiation of the isolate under analysis between monophasic Salmonella  Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula;

— identification of the isolate under analysis being either monophasic Salmonella  Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus
   Salmonella , isolated from: 

   
      
         

— products intended for human consumption;

         ...