ISO 14565:2000

Name: ISO 14565:2000 — Animal feeding stuffs — Determination of vitamin A content — Method using high-performance liquid chromatography
SKU: ISO 14565:2000
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Animal feeding stuffs — Determination of vitamin A content — Method using high-performance liquid chromatography

Название (английский):

ISO 14565:2000

Animal feeding stuffs — Determination of vitamin A content — Method using high-performance liquid chromatography

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 11
Дата публикации:: 7 декабря 2000 г.
Издание:: ISO IS 14565 edition 1 version 1
ICS:: 65.120

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Описание (английский):

La présente Norme internationale spécifie une méthode de détermination de la teneur en vitamine A totale (rétinol) des aliments pour animaux d'élevage et animaux de compagnie par chromatographie liquide à haute performance. La teneur en vitamine A est la teneur en alcool de rétinyle tout-trans et cis-isomères déterminée par la méthode décrite dans la présente Norme internationale, exprimée en Unités internationales par kilogramme (Ul/kg).

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 14565:2000 specifies a validated analytical method for determining total vitamin A (retinol) in animal feeding stuffs and pet foods using high-performance liquid chromatography (HPLC). The method quantifies all-trans-retinol and cis‑isomers and expresses results in International Units per kilogram (IU/kg). It is designed for routine laboratory use where accurate vitamin A content measurement is required for quality control, compliance and research.

Key topics and technical requirements

Scope & analyte: Total vitamin A (all‑trans‑retinol and cis‑isomers) in animal feeds and pet foods; results reported in IU/kg (1 IU vitamin A = 0.300 µg all‑trans‑retinol).

Principle: Sample saponification with ethanolic KOH, extraction into light petroleum, evaporation, dissolution in 2‑propanol and quantification by reverse‑phase HPLC producing a single retinol peak.

HPLC conditions & equipment highlights:

Column: C18, 250 mm × 4.6 mm, 5–10 µm particles, ≥4 000 theoretical plates, k' ≈ 0.6 for all‑trans‑retinol.
Mobile phase: methanol/water (770:30 by volume).
Flow rate: 1.0 ml/min; injection volume: 10 µl.
Detector: UV at 325 nm with integrator/data system.

Sample handling: Protect retinol from light and air (use amber glassware), work quickly and complete assays within one working day; store extracts under inert gas at 4 °C if delayed.

Calibration & standards: Hydrolysis of all‑trans‑retinyl acetate for calibration or use certified all‑trans‑retinol standards; spectrophotometric standardization at 325 nm using defined correction equations.

Expression of results: Calculate IU/kg from retinol concentration in extract and sample mass.

Precision: Interlaboratory precision data are provided (annex A) showing repeatability and reproducibility ranges across feed types.

Applications and users

Feed and pet food quality control laboratories verifying label claims and nutritional composition.

Manufacturers performing in‑house QC on premixes, concentrates and finished feeds.

Regulatory agencies and testing bodies assessing compliance with vitamin A specifications.

Research laboratories studying vitamin stability, bioavailability or formulation effects in feeds.

Related standards

ISO 3696:1987 - Water for analytical laboratory use (reagent water quality).

ISO 6498 - Preparation of test samples for animal feeding stuffs.

Annex A of ISO 14565 contains interlaboratory precision data for typical feed matrices.

Keywords: ISO 14565:2000, vitamin A determination, retinol, HPLC, animal feeding stuffs, feed testing, pet food analysis, saponification, IU/kg, C18 column.

ISO 14565:2000

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

ISO 3696:1987

Water for analytical laboratory use — Specification and test methods

ISO 6498:2012

Animal feeding stuffs — Guidelines for sample preparation

BS EN ISO 14565:2001

Animal feeding stuffs. Determination of vitamin A content. Method using high-performance liquid chromatograph…