ISO 15141:2018

Name: ISO 15141:2018 — Cereals and cereal products — Determination of ochratoxin A — High performance liquid chromatographic method with immunoaffinity column cleanup and fluorescence detection
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Cereals and cereal products — Determination of ochratoxin A — High performance liquid chromatographic method with immunoaffinity column cleanup and fluorescence detection

Название (английский):

ISO 15141:2018

Cereals and cereal products — Determination of ochratoxin A — High performance liquid chromatographic method with immunoaffinity column cleanup and fluorescence detection

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 12
Дата публикации:: 24 июля 2018 г.
Издание:: ISO IS 15141 edition 1 version 1
ICS:: 67.060

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Описание (английский):

This document specifies a high performance liquid chromatographic method with immunoaffinity column cleanup for the determination of ochratoxin A in cereals and cereal products. The limit of quantification is 0,2 μg/kg. The method detection limit is dependent on the sample matrix as well as on the instrument.

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты ISO

Overview

ISO 15141:2018 specifies a validated analytical procedure for the determination of ochratoxin A (OTA) in cereals and cereal products using high performance liquid chromatography (HPLC) with immunoaffinity column cleanup and fluorescence detection. The standard defines sample preparation, extraction, cleanup, HPLC conditions, calibration, identification and confirmation steps. The limit of quantification (LOQ) is 0.2 μg/kg; the method detection limit depends on sample matrix and instrument performance. ISO 15141:2018 replaces earlier ISO 15141-1/2 editions and refines extraction and purification principles.

Key Topics and Technical Requirements

Sample preparation and extraction

Typical sample mass: 25 g (12.5 g for light samples such as wheat bran).
Extraction solvent: acetonitrile–water (60:40, v/v); shake 30 min or blend 3 min; centrifuge or filter.

Dilution and cleanup

Dilution into phosphate buffered saline (PBS) and centrifugation/filtration options.
Immunoaffinity column containing anti‑OTA antibodies for selective cleanup (example product cited; equivalents allowed).
Column flow ~1–2 drops/s; wash with specified washing solution and water; elute with 1.5 ml methanol/acetic acid (98:2) and evaporate.

HPLC and detection

Reverse‑phase column (example: C18, 150 × 4.6 mm, 5 μm) for baseline resolution of OTA.
Mobile phase: acetonitrile/water/glacial acetic acid (48/51/1, v/v/v), degassed.
Fluorescence detection with optional confirmation by derivatization (boron trifluoride in methanol).

Calibration and performance

Calibration solutions covering 0.05–1.0 ng per 100 μl injection.
Precision data and interlaboratory results are included; laboratories must verify standard solution stability and instrument suitability.

Safety

OTA is nephrotoxic and a probable carcinogen - follow appropriate handling and decontamination procedures.

Applications and Users

ISO 15141:2018 is intended for:

Food testing and quality control laboratories analyzing cereals, flours, breakfast cereals, and related products.

Regulatory agencies enforcing mycotoxin limits and import/export control.

Agricultural processors and cereal manufacturers implementing HACCP and product safety monitoring.

Method developers and proficiency-test organisers who require a standardized, HPLC‑based OTA procedure.

Benefits include high selectivity from immunoaffinity cleanup, robust quantification at low μg/kg levels, and harmonized methodology to support compliance, risk assessment, and international trade.

ISO 15141:2018

Abstract

Похожие стандарты

Overview

Key Topics and Technical Requirements

Applications and Users

Related Standards

Технические детали

BS EN ISO 24333:2009

Cereals and cereal products. Sampling.

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