ISO 17410:2019

Name: ISO 17410:2019 — Microbiology of the food chain — Horizontal method for the enumeration of psychrotrophic microorganisms
SKU: ISO 17410:2019
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Microbiology of the food chain — Horizontal method for the enumeration of psychrotrophic microorganisms

Название (английский):

ISO 17410:2019

Microbiology of the food chain — Horizontal method for the enumeration of psychrotrophic microorganisms

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 10
Дата публикации:: 25 июля 2019 г.
Издание:: ISO IS 17410 edition 2 version 1
ICS:: 07.100.30

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Описание (английский):

This document specifies a horizontal method for the enumeration of psychrotrophic microorganisms that are able to grow and form colonies on a solid agar culture medium after aerobic incubation at 6,5 °C. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage. NOTE Annex B specifies a rapid method for the estimated enumeration of psychrotrophic microorganisms in raw and pasteurized milk.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 17410:2019 defines a horizontal method for the enumeration of psychrotrophic microorganisms in the food chain. The standard covers organisms able to form colonies on solid agar after aerobic incubation at 6.5 °C and applies to:

products intended for human consumption and animal feed

environmental samples from food and feed production and handling

samples from the primary production stage

The document specifies surface‑plating procedures (plate count agar), incubation, counting rules and reporting. Annex B provides a rapid estimation method for raw and pasteurized milk (incubation at 21 °C for 25 h), with the caveat that not all psychrotrophs detected at 6.5 °C will grow at 21 °C.

Key topics and technical requirements

Principle: Surface plating of a defined test portion or initial suspension, incubation at 6.5 °C ± 1 °C for 10 days, colony counting on plates with fewer than 150 colonies.

Sample handling: Prepare test samples and decimal dilutions per ISO 6887 series; ensure representative sampling and proper transport/storage.

Culture media and performance: Use Plate Count Agar (PCA); performance testing of media is required following ISO 11133 and Annex A.

Procedure details:

Dry plates before inoculation (per ISO 11133).
Inoculate 0.1 mL (or 1.0 mL on larger plates to increase sensitivity) and evenly spread across agar surface.
Incubate inverted plates at 6.5 °C for 10 days.
Count colonies (include pinpoint colonies) and calculate results as number of psychrotrophic microorganisms per gram or per millilitre.

Equipment: Incubator capable of 6.5 °C, sterile pipettes, spreaders, colony-counting device (optional), pH meter, refrigerator.

Quality assurance: Control plates, contamination checks and adherence to ISO 7218 general microbiology requirements.

Practical applications

ISO 17410:2019 is used for:

Quality control and shelf-life monitoring of refrigerated foods susceptible to psychrotrophic spoilage (dairy, meat, fish, ready-to-eat products).

Dairy industry testing, including monitoring raw and pasteurized milk for psychrotrophic plate counts (rapid method available).

Environmental monitoring in processing facilities to track refrigeration-related spoilage organisms.

Regulatory testing and supplier verification where consistent, comparable enumeration of psychrotrophic microbes is required.

Related standards

ISO 7218 - General requirements for microbiological examinations

ISO 6887 (all parts) - Preparation of test samples and dilutions

ISO 11133 - Culture media production and performance testing

ISO 835, ISO 8655 - Laboratory pipettes and volumetric apparatus

Keywords: ISO 17410:2019, psychrotrophic microorganisms, enumeration, plate count, surface plating, 6.5 °C incubation, plate count agar, food microbiology, rapid milk method.

ISO 17410:2019

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Practical applications

Who should use this standard

Related standards

Технические детали

ISO 6887-5:2020

Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for mi…

BS EN ISO 11133:2014+A2:2020

Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of cult…

ISO 7218:2007

Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examin…

ISO 835-3:1981

Laboratory glassware — Graduated pipettes — Part 3: Pipettes for which a waiting time of 15 s is specified

ISO 8655-2:2022

Piston-operated volumetric apparatus — Part 2: Pipettes