ISO 18862:2025

Overview

ISO 18862:2025 - "Coffee and coffee products - Determination of acrylamide" - specifies validated laboratory methods to measure acrylamide in coffee and coffee-derived products. The standard covers sample extraction with water, clean‑up by solid‑phase extraction (SPE) (and optional Carrez precipitation), and quantification by HPLC‑MS/MS or by GC‑MS after derivatization (bromination). Methods were validated across matrices including roasted coffee, soluble coffee, coffee substitutes and coffee products with a validated concentration range from 53 μg/kg to 612.1 μg/kg.

Key technical topics and requirements

• Extraction & clean‑up
  • Water extraction or dissolution for soluble products.
  • Matrix clean‑up using SPE; Carrez precipitation is included as an option to remove interferents.
• Analytical methods
  • HPLC‑MS/MS: direct measurement using tandem mass spectrometry.
  • GC‑MS after derivatization: requires bromination of acrylamide prior to GC‑MS analysis.
• Standards and calibration
  • Use of isotopically labelled internal standard (D3‑acrylamide; C‑13 labelled standard is allowed as an alternative).
  • Guidance on calibration, linearity, working range, laboratory recovery and measurement uncertainty.
• Reagents and safety
  • Specifies reagent grades (MS‑grade solvents, water per ISO 3696) and warns about acrylamide hazards - use fume hoods and protective measures.
• Validation and performance
  • Precision data (repeatability, reproducibility), recovery, and uncertainty guidance are provided.
  • Informative annexes include performance characteristics (Annex A), absorber materials (Annex B), example columns/conditions (Annex C) and HPLC‑MS/MS sample prep examples (Annex D).
• Documentation
  • Requirements for test reports and identification criteria for peak confirmation.

Practical applications and users

Who uses ISO 18862:2025:

• Food testing laboratories performing routine acrylamide monitoring in coffee.
• Coffee manufacturers and quality‑control teams validating product safety and process changes.
• Regulatory agencies and compliance bodies setting or enforcing acrylamide limits.
• Research labs studying acrylamide formation, mitigation strategies or processing effects.

Typical uses:

• Regulatory compliance testing and reporting.
• Internal quality control and supplier verification.
• Method transfer, proficiency testing and inter‑laboratory comparisons.
• Research into roasting/processing impacts on acrylamide levels.

Related standards

• ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
• The standard is the second edition and replaces ISO 18862:2016; consult national bodies for implementation and patent notices.

Keywords: ISO 18862:2025, acrylamide in coffee, HPLC‑MS/MS, GC‑MS derivatization, solid‑phase extraction (SPE), coffee product testing, acrylamide validation.