ISO 22992-2:2020

Overview

ISO 22992-2:2020 specifies a validated laboratory method for the determination of triclosan residues in textiles using liquid chromatography coupled with tandem mass spectrometry (HPLC‑/LC‑MS/MS). The procedure covers sample preparation, ultrasonic extraction with methanol, concentration and cleanup, instrumental analysis by LC‑MS/MS (electrospray ionization, MRM), calibration, blank control and result calculation. The method is applicable to all kinds of textile products.

Key topics and technical requirements

• Scope: Determination of triclosan (CAS 3380‑34‑5) in textile samples; applicable to all textile types.
• Sample preparation: Representative specimen cut to <5 mm pieces; typical test mass (1.00 ± 0.01) g.
• Extraction: Two-step ultrasonic extraction with methanol (25 mL each) at ≈40 °C for (30 ± 2) min; combined extracts concentrated with rotary evaporator to ≈1 mL, dried with nitrogen, reconstituted to 2 mL and filtered (0.22 µm).
• Standards & calibration: Stock standard 1 000 mg/L in methanol; working solutions prepared into at least five calibration levels; quantification by external standard method. Stock stored 0–4 °C up to 6 months; working solutions up to 3 months.
• LC‑MS/MS parameters (Annex A examples): C18 reversed‑phase column (150×2.1 mm, 3.5 µm); mobile phase methanol:water (90:10 v/v); flow 0.25 mL/min; injection 20 µL. ESI negative ion mode, MRM detection; typical precursor m/z 286.7 with transitions to m/z 35.0 (quant) and 142.1. Typical detection limit shown as 0.5 mg/kg.
• Quality control: Blank test to check contamination; dilution or increased sample mass recommended if analyte is outside linear range.
• Reporting & calculation: Results expressed as mg/kg (mass ratio triclosan/test specimen); report must reference ISO 22992‑2:2020 and note any deviations.

Practical applications and users

• Textile testing laboratories implementing regulatory or customer-driven chemical safety checks for triclosan in garments, home textiles and technical textiles.
• Manufacturers and suppliers verifying compliance, screening incoming materials or validating finishing processes that may use preservatives.
• Regulatory bodies and compliance auditors assessing conformity with chemical-restriction requirements.
• Research labs studying triclosan occurrence, migration or treatment effects in textile matrices.
  Keywords: ISO 22992-2:2020, triclosan residues, textiles, HPLC‑MS/MS, LC‑MS/MS, ultrasonic extraction, MRM.

Related standards

• ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
• ISO 22992 series - other parts address additional preservatives and methods (see ISO catalogue for full series).