ISO 24040:2022

Overview

ISO 24040:2022 specifies a laboratory method for the determination of certain benzotriazole UV-stabilizers (UV-320, UV-327, UV-328, UV-350) in textiles (fibres and fabrics). The standard covers sample preparation, solvent extraction, chromatographic analysis and calculation/reporting of results. Analytical options include LC-MS/MS, LC-DAD and GC-MS, with illustrative instrument parameters provided in informative annexes.

Key topics and technical requirements

• Scope: Quantitative determination of UV-320, UV-327, UV-328 and UV-350 in all kinds of textile materials.
• Sample preparation: Representative specimen cut to <5 mm pieces; weigh (1.00 ± 0.01) g.
• Extraction: Ultrasonic extraction using acetonitrile (two extractions of 15 ml at ~60 °C for 15 ± 1 min), combine extracts, concentrate nearly to dryness, reconstitute in 2 ml methanol, filter through 0.22 µm PTFE membrane.
• Reagents & materials: Analytical-grade methanol and acetonitrile, ammonium formate solution, Grade 2 water (ISO 3696), certified reference substances for UV-320/327/328/350.
• Instrumentation: Choice of LC-MS/MS (recommended with ESI positive and MRM), LC-DAD or GC-MS. Annexes provide example parameters (column type, gradients, MRM transitions, typical ions) to aid method setup.
• Calibration & quantification: At least five calibration points; example ranges: LC-MS/MS (0.5–2 µg/ml), LC-DAD/GC-MS (5–20 µg/ml). Blank runs required to control contamination. Calculation formulas convert measured concentration to mg/kg or % (accounting for blank).
• Performance: Example detection limit for LC-MS/MS reported at ~1 mg/kg under given conditions.
• Reporting: Test report must reference ISO 24040:2022, list test date, sample identification, detection method used, content of each benzotriazole compound and any deviations.

Applications and users

ISO 24040:2022 is intended for:

• Textile testing laboratories performing routine chemical safety and compliance testing.
• Manufacturers and quality control teams verifying raw materials or finished textiles for benzotriazole content.
• Regulatory bodies assessing chemical restrictions or monitoring hazardous additives in consumer textiles.
• Third‑party test houses and import/export compliance specialists needing harmonized, reproducible methods. Practical applications include product safety verification, restricted-substance screening, supply‑chain surveillance and environmental/occupational exposure studies involving textile materials.

Related standards

• ISO 3696 - Water for analytical laboratory use (referenced for Grade 2 water).
• Prepared by ISO/TC 38 (Textiles); consult national standards bodies for implementation guidance.

Keywords: ISO 24040:2022, benzotriazole, UV-320, UV-327, UV-328, UV-350, textiles testing, LC‑MS/MS, LC‑DAD, GC‑MS, ultrasonic extraction, acetonitrile.