ISO 24461:2022

Overview

ISO 24461:2022 specifies a laboratory test method to assess the anti-mosquito performance of textiles using an attractive blood feeding apparatus. The method evaluates a fabric’s ability to reduce mosquito contact and blood feeding whether or not the fabric is chemically treated. Tests are performed without human or animal blood-feeding sources and focus strictly on anti-mosquito performance (not disease prevention claims). Note: some elements of the method may be subject to patent rights.

Key topics and technical requirements

The standard defines the equipment, procedures and output metrics used to quantify fabric performance:

• Test principle

  • Use of an attractive blood feeding device to simulate mosquito attraction and feeding through the specimen.
  • Observation of landing, probing behaviour, and actual blood feeding by blood‑eager female mosquitoes.

• Apparatus and materials (main components)

  • Feeding device with feed reservoir and thermostat temperature regulator.
    • Feed reservoir feeding surface diameter: 35 mm ± 2 mm; capacity ≥ 3 ml.
    • Membranes: animal intestine/skin, collagen film, sausage casing or artificial skin (to be agreed by parties).
    • Thermostat control to ±0.5 °C.
  • Test cage: cuboid approx. 380 mm × 200 mm × 200 mm with mesh on five faces and three openings (diameter 55 mm ± 2 mm) connected to feed reservoirs.
  • Specimen holder and lid for quick removal and containment.
  • Frame to fix feeding surface at 45° from horizontal.
  • Carbon dioxide supply unit, ventilation fan and infrared surface thermometer (listed in the standard).
  • Test blood, test mosquitoes (blood-unfed females), and validation samples are specified.

• Procedure and outputs

  • Conditioning of specimens and defined test environment.
  • Separation and preparation of test mosquitoes and feed reservoir setup.
  • Calculations include landing rate, blood feeding rate, and blood feeding prevention index.
  • Validation checks and reporting requirements are provided.

Practical applications

• Product development and comparative performance testing of anti-mosquito fabrics (treated or structurally engineered).
• Quality control and batch verification in textile manufacturing.
• Independent lab testing for technical claims, marketing substantiation, or conformity assessment.
• Research into fabric treatments and structures that reduce mosquito contact and feeding.

Who should use this standard

• Textile manufacturers and R&D teams developing anti-mosquito products
• Independent textile testing laboratories and QA departments
• Regulatory and certification bodies assessing performance claims
• Product developers for outdoor apparel, bedding, nets and protective fabrics

Related information

• Prepared by ISO/TC 38 (Textiles). The document contains annexes on mosquito breeding environment, evaluation guidance, and mosquito age effects on feeding behaviour. Use keywords: ISO 24461, anti-mosquito fabric testing, attractive blood feeding apparatus, blood feeding prevention index, textile testing.