ISO 10272-1:2017

Name: ISO 10272-1:2017 — Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method
SKU: ISO 10272-1:2017
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Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method

Название (английский):

ISO 10272-1:2017

Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 24
Дата публикации:: 28 июня 2017 г.
Издание:: ISO IS 10272 edition 2 version 1
ICS:: 07.100.30

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Описание (английский):

ISO 10272-1:2017 specifies a horizontal method for the detection by enrichment or direct plating of Campylobacter spp. It is applicable to - products intended for human consumption, - products intended for animal feeding, - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage such as animal faeces, dust, and swabs.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 10272-1:2017 - "Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 1: Detection method" - specifies a validated, horizontal laboratory method for detection of Campylobacter spp. in food, feed and production environments. The standard covers detection by enrichment or direct plating, and applies to products intended for human consumption, animal feed, environmental samples in food/feed production and handling, and primary production samples (e.g., animal faeces, dust, swabs).

This second edition (2017) updates procedures to improve robustness against background flora (addition of Preston broth option), includes direct plating on mCCDA, and revises confirmation tests (study of aerobic growth at 25 °C and oxidase testing).

Key Topics

Scope and applicability: Foods, feeds, environmental and primary-production samples relevant to the food chain.

Detection procedures: Three detection procedures (A, B, C) using enrichment in selective liquid media or direct plating on selective solid media.

Selective media options: Inclusion of Bolton broth and an alternative (Preston broth) to address resistant background flora; direct plating on mCCDA as an option.

Isolation and confirmation: Isolation on selective agar followed by confirmation steps - colony selection, morphology and motility examination, oxidase activity, and aerobic growth assessment at 25 °C. Optional species identification includes catalase, hippurate and indoxyl acetate tests.

Performance and validation: The document defines performance characteristics (sensitivity, specificity, LOD) and includes interlaboratory study results and validation guidance in annexes.

Quality and safety: References for media preparation and performance testing (ISO 11133) and mandatory laboratory biosafety practices; work must be performed by skilled microbiologists in properly equipped labs.

Applications

Routine testing in food and feed microbiology laboratories for surveillance, batch release, and outbreak investigation.

Environmental monitoring in food processing plants and primary production farms (e.g., testing swabs, dust, and faecal material).

Regulatory compliance and verification testing by public health agencies and third-party auditors.

Method selection and laboratory accreditation where a harmonized, horizontal Campylobacter detection method is required.

Related standards

ISO 6887 (sample preparation)

ISO 7218 (general microbiological requirements)

ISO 11133 (culture media preparation and testing)

Other parts of the ISO 10272 series (enumeration methods and guidance)

Keywords: ISO 10272-1:2017, Campylobacter detection, food microbiology, enrichment method, mCCDA, Preston broth, Bolton broth, horizontal method.

ISO 10272-1:2017

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Who should use this standard

Related standards

Технические детали

BS EN ISO 11133:2014+A2:2020

Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of cult…

ISO 6887-5:2020

Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for mi…

ISO 7218:2007

Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examin…