ISO 16000-17:2008

Name: ISO 16000-17:2008 — Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method
SKU: ISO 16000-17:2008
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Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method

Название (английский):

ISO 16000-17:2008

Indoor air — Part 17: Detection and enumeration of moulds — Culture-based method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 27
Дата публикации:: 8 декабря 2008 г.
Издание:: ISO IS 16000 edition 1 version 1
ICS:: 13.040.20

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Срок поставки:5 раб. дн. (перевод)

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Описание (английский):

ISO 16000-17:2008 specifies a method for the detection and enumeration of moulds by cultivation after sampling by impaction according to ISO 16000-18:— or by filtration according to ISO 16000-16. It is also suitable for cultivation of moulds from material suspensions or from direct plating.

Abstract

Overview

Key topics and technical requirements

Sampling compatibility: Works with samples collected by filtration (filters) or impaction (agar impactor plates).
Sample processing: Filters are resuspended in saline (0.9% NaCl) with 0.01% polysorbate 80 (Tween), followed by decimal dilutions and plating (indirect method). Direct impaction plates are incubated without resuspension.
Culture media: Recommends DG18 (dichloran 18% glycerol agar) and either malt-extract agar or potato dextrose agar to recover a broad spectrum of indoor moulds. DG18 is used for its reduced water activity to support mould detection.
Incubation conditions: Standard incubation at (25 ± 3) °C. For targeted recovery of thermotolerant species, incubation at (36 ± 2) °C or (45 ± 2) °C (e.g., Aspergillus fumigatus) is described.
Examination and enumeration: After incubation, colonies are identified to the extent required and counted; results are calculated and reported as CFU per sampled volume or per unit area.
Equipment and quality: Specifies standard microbiological apparatus (vibration‑free incubators, safety cabinet, pH meter, autoclave) and use of validated reagents and media.
Safety: The standard includes a warning that hazardous materials and procedures may be involved; users must follow appropriate laboratory safety and regulatory requirements.

Applications and users

Indoor air quality (IAQ) assessments - building inspectors, IAQ consultants, occupational hygienists.
Microbiology and environmental laboratories - routine monitoring, diagnostic testing, and comparative analyses.
Remediation verification - confirm effectiveness of mould remediation or moisture control measures.
Research and epidemiology - studies linking indoor dampness/mould to health outcomes.
Regulatory and legal contexts - standardized evidence for compliance, risk assessment, or dispute resolution.

Keywords: ISO 16000-17:2008, indoor air, mould detection, culture-based method, DG18 agar, sampling by filtration, sampling by impaction, CFU, mould enumeration, indoor air quality.

Related standards

ISO 16000-16 - Detection and enumeration of moulds - Sampling by filtration.
ISO 16000-18 - Detection and enumeration of moulds - Sampling by impaction.
ISO 8199 - Guidance on enumeration of micro-organisms by culture (referenced for culturing practice).

Технические детали

Технический комитет: ISO/TC 146/SC 6 - Indoor air
SKU: ISO 16000-17:2008

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ISO 16000-17:2008

Abstract

Overview

Похожие стандарты

Key topics and technical requirements

Applications and users

Related standards

Технические детали

BS ISO 16000-16:2008

Indoor air. Detection and enumeration of moulds. Sampling by filtration.

BS ISO 16000-18:2011

Indoor air. Detection and enumeration of moulds. Sampling by impaction.

BS EN ISO 8199:2018

Water quality. General requirements and guidance for microbiological examinations by culture