ISO 8655-8:2022

Name: ISO 8655-8:2022 — Piston-operated volumetric apparatus — Part 8: Photometric reference measurement procedure for the determination of volume
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Piston-operated volumetric apparatus — Part 8: Photometric reference measurement procedure for the determination of volume

Название (английский):

ISO 8655-8:2022

Piston-operated volumetric apparatus — Part 8: Photometric reference measurement procedure for the determination of volume

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 18
Дата публикации:: 22 апреля 2022 г.
Издание:: ISO IS 8655 edition 1 version 1
ICS:: 17.060

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Описание (английский):

This document specifies the photometric reference measurement procedure for the determination of volume of piston-operated volumetric apparatus (POVA). The procedure is applicable to complete systems comprising the basic apparatus with a maximum nominal volume of 5 000 µl and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery (Ex).

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 8655-8:2022 specifies a photometric reference measurement procedure for the determination of delivered volume from piston-operated volumetric apparatus (POVA). The procedure covers complete systems - the basic apparatus and any selected disposable or reusable parts - with a maximum nominal volume of 5 000 µl, using a photometric (spectrophotometric) method to measure delivered liquid by delivery (Ex). It is part of the ISO 8655 series for piston-operated volumetric apparatus.

Key topics and requirements

Scope and applicability: Procedure applies to complete POVA systems (pipettes, dispensers, syringes, burettes as relevant) up to 5 000 µl and includes all parts used during delivery.

Test equipment: Specifies equipment such as spectrophotometers, cuvettes and mixers, measuring devices, balances, density meters and pH meters required for photometric volume determination.

Reagents and solutions: Identifies required reagents (e.g., buffer, Ponceau S, copper(II) chloride) and calibrator solutions and addresses solution stability, light sensitivity and storage.

Test conditions: Defines environmental and procedural test conditions (test room, evaporation control) to ensure repeatable photometric results.

Procedures:

System calibration and calibration-constant determination.
Photometric procedure including cuvette preparation, spectrophotometer zeroing, dispensing test liquids and measuring absorbance.
Specific test cycles for various device types (single- and multi-channel pipettes, positive-displacement pipettes, burettes, dispensers, syringes) in accordance with related ISO 8655 parts.

Evaluation and reporting: Calculation of delivered volume, temperature correction, mean volume, systematic and random error analysis, and measurement uncertainty. Reporting requirements and referencing normative annexes (e.g., balance-based volume calculations).

Applications and users

Calibration laboratories and test houses seeking a traceable, high-precision method for volumetric calibration using photometry.

Manufacturers of pipettes and piston-operated devices for quality control and declaration of performance.

Laboratory quality managers and users who require validated calibration methods for routine verification and instrument qualification.

Useful in regulated environments (e.g., clinical, pharmaceutical, research) where accurate micro-volume delivery (µl range) must be demonstrated.

Related standards

ISO 8655 series (Part 1 general requirements; Part 2 pipettes; Part 3 burettes; Part 5 dispensers; Part 9 syringes)

ISO 1042 (volumetric flasks) and ISO 3696 (water for analytical use)

ISO/IEC Guide 99 (VIM) for metrology vocabulary

Keywords: ISO 8655-8:2022, photometric reference measurement procedure, piston-operated volumetric apparatus, pipette calibration, spectrophotometric volume determination, measurement uncertainty.

ISO 8655-8:2022

Abstract

Похожие стандарты

Overview

Key topics and requirements

Applications and users

Related standards

Технические детали

ISO 8655-2:2022

Piston-operated volumetric apparatus — Part 2: Pipettes

ISO 10426-2:2003

Petroleum and natural gas industries — Cements and materials for well cementing — Part 2: Testing of well cem…

BS EN ISO 3696:1995

Water for analytical laboratory use. Specification and test methods.