ISO/TS 16782:2016

Overview

ISO/TS 16782:2016 - Clinical laboratory testing - Criteria for acceptable lots of dehydrated Mueller‑Hinton agar and broth for antimicrobial susceptibility testing - specifies how manufacturers and users assess production lots of dehydrated Mueller‑Hinton broth (dMHB) and dehydrated Mueller‑Hinton agar (dMHA) for use in antimicrobial susceptibility testing (AST). The Technical Specification defines physical and chemical characteristics, performance criteria, and lot‑testing protocols so that dMHB/dMHA lots can be reliably used for AST methods (notably disk diffusion and broth microdilution).

Key phrases: ISO/TS 16782:2016, dehydrated Mueller‑Hinton agar, dehydrated Mueller‑Hinton broth, antimicrobial susceptibility testing, dMHA, dMHB.

Key Topics

• Scope and exclusions
  • Covers physical properties and performance criteria for dehydrated MH media lots.
  • Excludes supplements (e.g., blood or blood products) added later to support fastidious organisms.
  • For dMHA, performance testing in this TS is limited to disk diffusion as described by CLSI and EUCAST.
• Physical and chemical specifications
  • Prepared MHB: pH 7.2–7.4 (25 °C); light straw coloured, clear, no precipitate.
  • Dehydrated powder: uniform, free‑flowing, beige to light beige.
• Cation content and supplementation
  • Cation concentrations influence MICs and disk diffusion-critical for agents such as aminoglycosides and quinolones.
  • Typical final prepared MHB targets: 20–25 mg/L Ca and 10–12.5 mg/L Mg (production lots ≤25 mg/L Ca and ≤12.5 mg/L Mg; manufacturers may label actual levels if