ISO/TS 6579-2:2012
Microbiology of food and animal feed — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 2: Enumeration by a miniaturized most probable number technique
Microbiology of food and animal feed — Horizontal method for the detection, enumeration and serotyping of Salmonella — Part 2: Enumeration by a miniaturized most probable number technique
- Статус документа:
- Действующий
- Формат:
- Электронный (PDF)
- Количество страниц:
- 18
- Дата публикации:
- 5 ноября 2012 г.
- Издание:
- ISO TS 6579 edition 1 version 1
- ICS:
- 07.100.30
ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (
Abstract
Overview
ISO/TS 6579-2:2012 specifies a horizontal laboratory method for the enumeration of Salmonella spp. in food, animal feed, environmental samples (production, handling, primary production) and animal faeces using a miniaturized Most Probable Number (MPN) technique. The procedure miniaturizes dilution, pre-enrichment and selective enrichment steps to provide quantitative estimates of Salmonella concentration per gram or millilitre. The method employs buffered peptone water (BPW) for pre-enrichment, modified semi-solid Rappaport–Vassiliadis (MSRV) for selective enrichment (targeting motile salmonellae) and XLD for selective plating, followed by biochemical and serological confirmation and calculation of the MPN.
Key topics and technical requirements
- Scope and limitations
- Applies to products for human and animal consumption, environmental and faecal samples.
- Not suitable for samples with very low contamination (
Технические детали
- Технический комитет
- ISO/TC 34/SC 9 - Microbiology
- SKU
- ISO/TS 6579-2:2012
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