PD CEN ISO/TS 6579-2:2012

1   Scope This part of ISO 6579 gives a method for the enumeration of Salmonella spp. present in:

— products intended for human consumption and for the feeding of animals;

— environmental samples in the area of food production and food handling;

— animal faeces;

— environmental samples from the primary production stage;

by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport–Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).

NOTE The number of non-motile salmonellae is generally low in most of the matrices relevant for this method. In this n...