SIST EN ISO 16266:2008

Name: SIST EN ISO 16266:2008 — Water quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration (ISO 16266:2006)
SKU: SIST EN ISO 16266:2008
Price: 34200 RUB
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Water quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration (ISO 16266:2006)

Название (английский):

Water quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration (ISO 16266:2006)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 19
Дата публикации:: 6 июня 2008 г.
ICS:: 13.060.70
Технический комитет:: KAV - Water quality

Тип поставки:

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Цена:34 200 ₽

Описание (английский):

ISO 16266:2006 specifies a method for the isolation and enumeration of Pseudomonas aeruginosa in samples of bottled water by a membrane filtration technique. This method can also be applied to other types of water with a low background flora, for example, pool waters and waters intended for human consumption.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

EN ISO 16266:2008 (ISO 16266:2006) specifies a standardized membrane filtration method for the detection and enumeration of Pseudomonas aeruginosa in bottled water and other waters with low background flora (for example, pool waters and waters intended for human consumption). The standard describes sampling volumes, culture media, incubation, colony enumeration and confirmation tests to reliably identify P. aeruginosa for water quality and public-health monitoring.

Key Topics and Requirements

Principle: Filter a measured volume (or dilution) through a 0.45 µm membrane filter, place the filter onto selective medium, incubate and count characteristic colonies.

Target organism definition: Colonies producing pyocyanin, fluorescing under UV (360 ± 20 nm), oxidase-positive and able to produce ammonia from acetamide are considered P. aeruginosa.

Selective medium: Pseudomonas agar base (CN-agar) with CN supplement (cetrimide 0.2 g/L and nalidixic acid 0.015 g/L) is specified; detailed preparation, pH and storage conditions are given.

Confirmation steps: Subculture suspicious colonies onto nutrient agar or King’s B; perform oxidase test, fluorescence checks and acetamide utilization (acetamide broth) to confirm identity.

Laboratory practice: Media preparation (autoclave at 121 ± 3 °C for 15 min), use of analytical-grade reagents, incubation conditions, and plate storage limits are specified.

Sampling guidance: Typical regulatory expectations cited - bottled and spring waters should be free of P. aeruginosa in any 250 ml sample; other waters often tested in 100 ml volumes.

Quality assurance & safety: Tests must be carried out by trained personnel; precautions for handling hazardous reagents (e.g., acetamide) and following safety and QA procedures are emphasized.

Applications and Who Uses It

Water testing laboratories performing routine microbiological analysis for bottled water, municipal supply or recreational waters.

Bottled water and spring water producers confirming compliance with regulatory limits (absence in 250 ml).

Public health and regulatory authorities evaluating water safety and enforcing standards.

Pool operators and facility managers monitoring recreational waters with low background flora.

Manufacturers of water treatment and packaging equipment needing validated microbiological methods for product verification.

Related Standards

ISO 3696 (water for laboratory use), ISO 5667 (sampling guidance), ISO 7704 (membrane filters), ISO 8199 (enumeration guidance), ISO 19458 (microbial sampling). EN ISO 16266:2008 supersedes EN 12780:2002.

Keywords: EN ISO 16266:2008, ISO 16266, Pseudomonas aeruginosa detection, membrane filtration method, water quality testing, bottled water microbiology.

SIST EN ISO 16266:2008

Abstract

Похожие стандарты

Overview

Key Topics and Requirements

Applications and Who Uses It

Related Standards

Технические детали

ISO 16266:2006

Water quality — Detection and enumeration of Pseudomonas aeruginosa — Method by membrane filtration

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

BS ISO 5667-21:2010

Water quality. Sampling - Guidance on sampling of drinking water distributed by tankers or means other than d…

SIST EN ISO 7704:2023

Water quality - Requirements for the performance testing of membrane filters used for direct enumeration of m…

SIST EN ISO 8199:2019

Water quality - General requirements and guidance for microbiological examinations by culture (ISO 8199:2018)

SIST EN ISO 19458:2007

Water quality - Sampling for microbiological analysis (ISO 19458:2006)

BS EN ISO 16266:2008

Water quality. Detection and enumeration of Pseudomonas aeruginosa. Method by membrane filtration.