CEN ISO/TS 29843-1:2014 PDF

Name: CEN ISO/TS 29843-1:2014 — Soil quality - Determination of soil microbial diversity - Part 1: Method by phospholipid fatty acid analysis (PLFA) and phospholipid ether lipids (PLEL) analysis (ISO/TS 29843-1:2010)
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Soil quality - Determination of soil microbial diversity - Part 1: Method by phospholipid fatty acid analysis (PLFA) and phospholipid ether lipids (PLEL) analysis (ISO/TS 29843-1:2010)

Название (английский):

CEN ISO/TS 29843-1:2014

Soil quality - Determination of soil microbial diversity - Part 1: Method by phospholipid fatty acid analysis (PLFA) and phospholipid ether lipids (PLEL) analysis (ISO/TS 29843-1:2010)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 17
Дата публикации:: 22 октября 2014 г.
Издание:: CEN TS 29843 edition 1 version 1
ICS:: 13.080.30

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Описание (английский):

ISO/TS 29843‑1:2010 specifies an extended method for the extraction and determination of both phospholipid fatty acids (PLFA) and phospholipid ether lipids (PLEL) from soils. ISO/TS 29843‑2 specifies a simple method for the extraction of only PLFA from soils.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

CEN ISO/TS 29843-1:2014 (identical to ISO/TS 29843-1:2010) is a technical specification for assessing soil quality by determining soil microbial diversity using an extended lipid-based analytical approach. The standard specifies a method to extract and quantify both phospholipid fatty acids (PLFA) and phospholipid ether lipids (PLEL) from soils. PLFA/PLEL profiling provides a quantitative measure of viable microbial biomass and a phenotypic fingerprint for taxonomic differentiation within complex soil microbial communities.

Key Topics

Scope: Defines an extended extraction and analysis method for both PLFA and PLEL; Part 2 of the series covers a simpler PLFA-only extraction.

Sampling guidance: Refers to ISO 10381-6 for collection, handling and storage of soil intended for microbiological and biomass analyses.

Extraction principle: Lipids are extracted using the Bligh and Dyer procedure and separated on a silica (si-) column.

Transformations and separations:

Mild alkaline hydrolysis to convert phospholipids to fatty acid methyl esters (FAME).
Acidic methylation for PLEL conversion and specific cleavage of ether bonds (e.g., hydroiodic acid, HI, and subsequent reductive steps) as described in the text.
Use of solid-phase extraction (SPE) columns (NH, SCX and others) to fractionate saturated (SATFA), mono-unsaturated (MUFA), poly-unsaturated (PUFA), hydroxy-substituted (PLOH) and non-ester-linked fatty acids.
Derivatization procedures (e.g., TMSI, DMDS) and GC/MS measurement for identification and quantification.

Measurement & calculation: Procedures for GC/MS-based identification and calculation of PLFA/PLEL fractions to infer microbial composition and physiological state.

Applications

Environmental and soil quality assessments that require reliable measures of microbial biomass and community composition.

Soil ecology and agronomy studies tracking microbial responses to land use, pollution, remediation, fertilization, or climate-related stress.

Environmental monitoring and research laboratories needing standardized, reproducible PLFA/PLEL analysis.

Complementary use alongside molecular/genetic methods for a combined phenotypic and genotypic view of soil microbial diversity.

Related Standards (if applicable)

ISO/TS 29843-2 - Simple PLFA extraction method (PLFA-only).

ISO 10381-6 - Guidance on soil sampling, handling and storage for microbiological assessments.

Keywords: soil quality, microbial diversity, PLFA, PLEL, phospholipid fatty acid analysis, lipid extraction, Bligh and Dyer, GC/MS, ISO/TS 29843-1, CEN ISO/TS 29843-1.

CEN ISO/TS 29843-1:2014 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related Standards (if applicable)

Технические детали

ISO 10381-6:1993

Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil for the assessment…

SIST-TS CEN ISO/TS 29843-2:2021

Soil quality - Determination of soil microbial diversity - Part 2: Method by phospholipid fatty acid analysis…

SIST-TS CEN ISO/TS 29843-1:2015

Soil quality - Determination of soil microbial diversity - Part 1: Method by phospholipid fatty acid analysis…