Overview
EN ISO 14182:1999 (ISO 14182:1999) specifies a validated gas chromatographic method for the determination of residues of selected organophosphorus pesticides in animal feeding stuffs. Developed by ISO/TC 34 SC10 and adopted by CEN, the method covers extraction, cleanup and instrumental determination using a phosphorus-selective detector (FPD/NPD in P mode) or mass-selective detector (GC–MS). The method’s reported lower limit of determination is 0.01 µg/g for the listed pesticides.
Key topics and technical requirements
- Target analytes: azinphos-ethyl, azinphos-methyl, bromophos, carbophenothion, chlorpyrifos (and methyl), diazinon, dimethoate, ethion, fonofos, malathion, methidathion, parathion (and methyl), pirimiphos-ethyl and pirimiphos-methyl. (Note: other organophosphorus compounds may be amenable but are not validated.)
- Sample extraction & cleanup: test portion extracted with acetone, diluted with water/saturated NaCl and partitioned into dichloromethane; concentrate and purify on a 10% water‑deactivated silica gel column.
- Instrumentation: Gas chromatography with splitless/on‑column injection; capillary or packed columns with appropriate stationary phases; detectors-phosphorus‑selective (FPD/NPD P‑mode) or MSD with minimum sensitivity around 50 pg P-compounds.
- Validation & QC: system linearity check (example given with parathion from 0.1–2 ng), calibration using internal standard tributyl phosphate, blank checks, and interlaboratory performance data (informative Annex B).
- Procedural references: sample preparation per ISO 6498, water quality per ISO 3696; sampling guidance is referenced (ISO 6497).
Applications and users
- Who uses it: analytical laboratories, feed manufacturers’ QC teams, regulatory agencies and enforcement laboratories performing pesticide residue analysis in animal feed.
- Practical uses:
- Routine monitoring for compliance with residue limits.
- Method transfer and validation for laboratories implementing GC–FPD or GC–MS pesticide assays.
- Comparative studies and interlaboratory proficiency testing.
Related standards
- ISO 3696 - Water for analytical laboratory use
- ISO 6498 - Preparation of test samples (animal feeding stuffs)
- ISO 6497 - Recommended sampling methods (referenced)
- EN adoption: this International Standard is published as EN ISO 14182:1999 (SIST EN ISO 14182:2000 in Slovenia).
This standard is a practical reference for labs implementing gas chromatographic pesticide residue methods in feed matrices and supports robust QA, instrument setup, and validated reporting for organophosphorus pesticide surveillance. Keywords: EN ISO 14182:1999, organophosphorus pesticides, animal feeding stuffs, gas chromatographic method, pesticide residue analysis, GC‑MS, GC‑FPD.