EN ISO 15181-6:2014 PDF

Overview

EN ISO 15181-6:2014 (ISO 15181-6:2012) specifies a laboratory method for determining the release rate of the biocide tralopyril from antifouling paints. Because tralopyril is hydrolytically unstable in seawater, the standard measures its stable degradation product - 3-bromo-5-(4-chlorophenyl)-4-cyano-1H-pyrrole-2-carboxylic acid (BCCPCA) - after accelerating conversion by heat treatment. The measured BCCPCA concentration in an artificial seawater extract (prepared according to ISO 15181-1) is used to calculate the tralopyril release rate under standardized laboratory conditions.

Key topics and requirements

• Scope and principle: quantitation of BCCPCA as a surrogate for released tralopyril; conversion by controlled heat treatment and subsequent instrumental analysis.
• Sample preparation: extraction of biocides into artificial seawater following ISO 15181-1, then sub-sampling and heat-induced conversion to BCCPCA.
• Analytical procedure: preparation of calibration standards, instrument calibration, recovery and conversion checks, and sample determination (chromatographic/analytical quantitation of BCCPCA).
• Calculations: conversion of measured BCCPCA concentration to tralopyril release rate per cylinder and per area, cumulative release, mean and pseudo‑steady‑state release rates.
• Performance limits: when used with ISO 15181-1 the practical quantitation range for release rates is 0.36 µg·cm⁻²·d⁻¹ to 270 µg·cm⁻²·d⁻¹. Quantitation of lower release rates requires an analytical method with a limit of quantitation for tralopyril in artificial seawater of **