EN ISO 15302:2017 PDF

Name: EN ISO 15302:2017 — Animal and vegetable fats and oils - Determination of benzo[a]pyrene - Reverse-phase high performance liquid chromatography method (ISO 15302:2017)
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Animal and vegetable fats and oils - Determination of benzo[a]pyrene - Reverse-phase high performance liquid chromatography method (ISO 15302:2017)

Название (английский):

EN ISO 15302:2017

Animal and vegetable fats and oils - Determination of benzo[a]pyrene - Reverse-phase high performance liquid chromatography method (ISO 15302:2017)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 16
Дата публикации:: 26 апреля 2017 г.
Издание:: CEN EN 15302 edition 2 version 1
ICS:: 67.200.10

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Описание (английский):

ISO 15302:2017 specifies a method for the determination of benzo[a]pyrene in crude or refined edible oils and fats by reverse-phase high performance liquid chromatography (HPLC) using fluorimetric detection in the range 0,1 µg/kg to 50 µg/kg. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Abstract

Похожие стандарты

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Overview

EN ISO 15302:2017 (CEN endorsement of ISO 15302:2017) defines a laboratory method for the determination of benzo[a]pyrene in crude or refined edible animal and vegetable fats and oils. The standard specifies a reverse‑phase high performance liquid chromatography (HPLC) method with fluorimetric detection and covers the analytical range 0.1 µg/kg to 50 µg/kg. Milk and milk products (and fats derived from them) are excluded from the scope.

Key topics and technical requirements

Analytical principle: Sample dissolution in light petroleum, addition of an internal standard (benzo[b]chrysene), adsorption on alumina and cleanup, followed by reverse‑phase HPLC with fluorimetric detection.

Detection range: Method validated for benzo[a]pyrene mass fractions from 0.1 µg/kg to 50 µg/kg.

Reagents: Specifies analytical‑grade solvents and reagents such as light petroleum or hexane, acetonitrile, tetrahydrofuran, toluene, sodium sulfate and deactivated alumina. Preparation of benzo[a]pyrene stock and working standards is described.

Internal standard: Use of benzo[b]chrysene as the internal standard for quantification.

Apparatus: Typical lab equipment plus HPLC system with injection valve (50 µl loop or autosampler), reverse‑phase guard and analytical columns for polycyclic aromatic hydrocarbons (PAHs), water baths, alumina columns and a flash/rotary evaporator.

Sample preparation & sampling: Refers to ISO 661 for preparation of test samples; provides procedures for adsorption, elution and concentration prior to HPLC analysis.

Quality and performance: Sections on calculation of relative response factors, expression of results, and precision (repeatability and reproducibility) are included.

Safety: Explicit warnings on the carcinogenic nature of benzo[a]pyrene and safe handling (fume hood, gloves).

Applications and users

Food testing laboratories performing benzo[a]pyrene and PAH analysis in edible oils and fats.

Quality control teams and analytical chemists in edible oil processing and refining (vegetable oils, animal fats).

Regulatory authorities and compliance labs verifying limits for carcinogenic PAHs in food products.

Research groups studying contamination, processing effects, or mitigation strategies for PAHs in oils.

Practical benefits:

Provides a standardized, traceable HPLC‑based procedure for low‑level benzo[a]pyrene quantification.

Enables comparable results across laboratories for regulatory and safety assessments.

Related standards

ISO 661 - Preparation of test sample for animal and vegetable fats and oils (normative reference cited in EN ISO 15302:2017).

Linked technical committees: ISO/TC 34 (Food products) and CEN/TC 307 (Oilseeds, vegetable and animal fats and oils).

Keywords: EN ISO 15302:2017, benzo[a]pyrene, reverse‑phase HPLC, fluorimetric detection, edible oils, animal and vegetable fats, PAH analysis, food safety, analytical method.

EN ISO 15302:2017 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

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