EN ISO 16187:2025 PDF

Overview

EN ISO 16187:2025 (ISO 16187:2025) specifies quantitative laboratory test methods to assess the antibacterial activity of footwear and footwear components. Adopted by CEN as EN ISO 16187:2025, the standard applies to all types of footwear and components that use non‑diffusing antibacterial treatments. It replaces EN ISO 16187:2013 / ISO 16187:2013 and defines procedures for measuring and reporting antibacterial performance in a reproducible way.

Key topics and requirements

• Scope and safety: The document includes a clear safety caution - tests use live bacteria and must be carried out in appropriate containment facilities by trained personnel.
• Test principles: Quantitative evaluation of antibacterial activity using controlled inocula and contact between microorganisms and the treated material.
• Test methods (normative annexes):
  • Static challenge test (Annex A)
  • Film contact method (Annex B)
  • Dynamic challenge test (Annex C)
• Test setup components:
  • Apparatus and environmental conditions
  • Reagents and culture media (e.g., nutrient broth/agar; alternatives such as tryptic soy media are noted)
  • Test microorganisms (specified strains and storage)
  • Preparation of inocula and test specimens, including pre‑treatment
• Test procedure and result expression:
  • Enumeration of viable bacteria and calculation methods
  • Antibacterial activity ratio and criteria for judging test effectiveness
  • Required content for the test report
• Terminology and updates: The 2025 edition adds terms (e.g., “neutralizer”), updates media options (TSA/TSB alternatives), and revises normative references.

Practical applications and users

Who uses ISO 16187:2025:

• Footwear manufacturers developing or validating antibacterial treatments (insoles, linings, uppers)
• Independent testing laboratories performing product certification, quality control, or comparative testing
• R&D teams evaluating new antimicrobial treatments and formulations
• Procurement and regulatory bodies requiring documented antibacterial performance for specifications or claims
• Standards bodies and conformity assessors

Typical uses:

• Verifying antibacterial performance claims
• Routine quality control during production
• Comparing treatment options in product development
• Supporting compliance for market access and technical specifications

Related standards

• ISO/TC 216 (Footwear) and CEN/TC 309 (Footwear) collaboration
• Supersedes ISO 16187:2013 / EN ISO 16187:2013

Keywords: ISO 16187, EN ISO 16187:2025, antibacterial footwear test, footwear antibacterial activity, static challenge test, film contact method, dynamic challenge test, non‑diffusing antibacterial treatments.