ISO 10315:2021 PDF

Name: ISO 10315:2021 — Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-chromatographic method
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Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-chromatographic method

Название (английский):

ISO 10315:2021

Cigarettes — Determination of nicotine in total particulate matter from the mainstream smoke — Gas-chromatographic method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 14
Дата публикации:: 1 июня 2021 г.
Издание:: ISO IS 10315 edition 4 version 1
ICS:: 65.160

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Описание (английский):

This document specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISO 4387. NOTE ISO 20778 and ISO 22253 provide the determination method of nicotine in smoke with an intense smoking regime.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 10315:2021 specifies a gas‑chromatographic method for the determination of nicotine in total particulate matter (TPM) collected from the mainstream smoke of cigarettes. Smoking and TPM collection are performed in accordance with ISO 4387. The method dissolves the TPM in an extraction solution containing an internal standard, separates nicotine by gas chromatography with a flame ionization detector (FID), and quantifies nicotine by calibration against reference solutions.

Key topics and technical requirements

Scope: Quantitative determination of nicotine in TPM from mainstream cigarette smoke using GC‑FID.

Sampling basis: TPM obtained using the ISO 4387 machine‑smoking regime. (Note: ISO 20778 and ISO 22253 cover intense smoking regimes.)

Extraction: TPM dissolved in propan‑2‑ol containing an internal standard (typically n‑heptadecane or quinaldine), with recommended extraction volumes (e.g., 20 ml for 44 mm discs, 50 ml for 92 mm discs).

Calibration: Series of at least four calibration solutions covering the expected nicotine range (commonly 0.02 mg/ml to 2.0 mg/ml); linear regression of area ratios (nicotine/internal standard) is used for quantitation.

GC conditions (example operating parameters):

Column temperature: 170 °C (isothermal)
Injection temperature: 250 °C; detector (FID) temperature: 250 °C
Carrier gas: helium, nitrogen, or hydrogen (~30 ml/min)
Injection volume: 2 µl
Typical analysis time: ~6–8 minutes

Reagents and reference materials: High‑purity carrier gases, propan‑2‑ol (low water content), nicotine reference substance (≥98 % purity) stored at 0–4 °C and protected from light; internal standards at 0.2–0.5 mg/ml in extraction solution.

Quality controls: Monitor internal standard peak consistency; allow refrigerated solutions to equilibrate to ambient temperature before use; follow documented calibration and reporting procedures.

Applications and users

This standard is intended for:

Tobacco testing laboratories performing routine product characterization and quality control.

Manufacturers assessing nicotine yields from cigarette designs during development.

Regulatory bodies and compliance labs requiring standardized analytical methods for emissions reporting.

Academic and industry researchers studying mainstream smoke composition and product comparisons.

Practical benefits include reproducible nicotine measurement in TPM, compatibility with standard machine‑smoking protocols, and integration with complementary analyses (e.g., water determination by ISO 10362‑1).

Related standards

ISO 4387 - TPM collection and machine smoking

ISO 20778, ISO 22253 - nicotine determination with intense smoking regimes

ISO 3400 - total nicotine alkaloids (alternative method)

ISO 10362‑1 - GC method for water determination in smoke

Additional standards in the ISO/TC 126 set (ISO 3308, ISO 3402, ISO 8243)

Keywords: ISO 10315:2021, nicotine determination, gas chromatographic method, total particulate matter, mainstream smoke, cigarette testing, GC‑FID, tobacco analytical standard.

ISO 10315:2021 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

SIST ISO 4387:2020

Cigarettes - Determination of total and nicotine-free dry particulate matter using a routine analytical smoki…

BS ISO 20778:2018

Cigarettes. Routine analytical cigarette smoking machine. Definitions and standard conditions with an intense…

BS ISO 22253:2019

Cigarettes. Determination of nicotine in total particulate matter from the mainstream smoke with an intense s…

SIST ISO 10362-1:2020

Cigarettes - Determination of water in total particulate matter from the mainstream smoke

ISO 3400:1997/Amd 1:2009

Cigarettes — Determination of alkaloids in smoke condensates — Spectrometric method — Amendment 1

SIST ISO 3308:2014

Routine analytical cigarette-smoking machine - Definitions and standard conditions

SIST ISO 3402:2023

Tobacco and tobacco products - Atmosphere for conditioning and testing

SIST ISO 8243:2014

Cigarettes - Sampling