ISO 10540-2:2003 PDF

Overview - ISO 10540-2:2003 (Phosphorus in fats and oils)

ISO 10540-2:2003 specifies a rapid, trace-level method for the determination of phosphorus in crude or refined animal and vegetable fats and oils using graphite furnace atomic absorption spectrometry (GFAAS). The method is applicable for phosphorus contents up to 40 mg/kg, with a stated limit of determination of 1.0 mg/kg. It is part of the ISO 10540 series on phosphorus determination in fats and oils.

Key topics and technical requirements

• Scope: Trace phosphorus (≤ 40 mg/kg) in animal and vegetable fats and oils; limit of determination 1.0 mg/kg.
• Analytical principle: Sample mixed with a matrix modifier and vaporized in a graphite furnace; phosphorus measured by atomic absorption at 213.5 nm.
• Reagents & standards:
  • Cyclohexane as solvent.
  • Lanthanum organometallic matrix modifier (lanthanum essential to measure total phosphorus reliably).
  • Soya lecithin used to prepare stock and working standards (typical working concentrations: 10, 20 and 40 mg/kg; stock ~400 mg/kg).
  • Blank oil (refined edible oil