ISO 10932:2010 PDF

Name: ISO 10932:2010 — Milk and milk products — Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB)
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Milk and milk products — Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB)

Название (английский):

ISO 10932:2010

Milk and milk products — Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 31
Дата публикации:: 7 июня 2010 г.
Издание:: ISO IS 10932 edition 1 version 1
ICS:: 67.100.01

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Описание (английский):

ISO 10932|IDF 223:2010 specifies a method for determining the minimal inhibitory concentration (MIC) of a series of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB). ISO 10932|IDF 223:2010 recommends the broth microdilution method as the standard method.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 10932:2010 (IDF 223:2010) defines a standardized method for determining the minimal inhibitory concentration (MIC) of antibiotics for bifidobacteria and non-enterococcal lactic acid bacteria (LAB) isolated from milk and milk products. The standard recommends the broth microdilution method as the reference quantitative technique: the MIC is the lowest antibiotic concentration that prevents visible growth under defined in vitro conditions. The document includes procedural steps, media formulations, quality control and reporting requirements relevant to dairy microbiology and probiotic testing.

Key Topics

Scope and principle

Quantitative MIC determination for bifidobacteria and non‑enterococcal LAB using broth microdilution.
Definition of MIC (expressed in µg/ml) and the rationale for standardization.

Culture media and reagents

Specification and preparation guidance for recommended media such as MRS agar, MRS-Cys, and M17‑sucrose to avoid media–antibiotic antagonism.
Use of analytical-grade reagents, sterile water and pre-reduced media where required.

Procedure and test setup

Colony selection and suspension, propagation, dilution, and inoculation of microdilution plates with two‑fold antibiotic dilution series.
Growth conditions and incubation parameters, preparation of microdilution plates (including hand-made options), and criteria for visible growth assessment.

Quality control and performance

Inclusion of control strains, interlaboratory precision (repeatability and reproducibility) data, and validation of media performance.

Results and reporting

How to express MIC values, document test conditions, and report results for regulatory or product‑development use.

Safety note

Warnings on hazards associated with handling antibiotics (e.g., kanamycin, chloramphenicol) and recommended precautions.

Applications

ISO 10932:2010 is directly applicable to:

Dairy microbiology laboratories testing milk, fermented products and probiotic cultures for antibiotic susceptibility.

Probiotic and starter-culture manufacturers evaluating strain safety and antibiotic resistance profiles.

Regulatory agencies and accreditation bodies assessing compliance and harmonizing susceptibility testing methods.

Research groups studying antimicrobial resistance in LAB from dairy ecosystems.

Benefits include reproducible, comparable MIC data for risk assessment, product development, and surveillance of antibiotic resistance in dairy-associated microorganisms.

Related standards

ISO 6887-5 - sample preparation for milk and milk products

ISO 7218 - general microbiological examination requirements

ISO/TS 11133-1 and -2 - preparation and performance testing of culture media

ISO 20776-1 - terminology and definitions relating to MIC

Keywords: ISO 10932:2010, MIC determination, broth microdilution, bifidobacteria, lactic acid bacteria, LAB, milk and milk products, MRS agar, antibiotic susceptibility.

ISO 10932:2010 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related standards

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