ISO 11868:2007 PDF

Name: ISO 11868:2007 — Heat-treated milk — Determination of lactulose content — Method using high-performance liquid chromatography
SKU: ISO 11868:2007
Price: 25 EUR
Availability: InStock

Heat-treated milk — Determination of lactulose content — Method using high-performance liquid chromatography

Название (английский):

ISO 11868:2007

Heat-treated milk — Determination of lactulose content — Method using high-performance liquid chromatography

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 10
Дата публикации:: 9 марта 2007 г.
Издание:: ISO IS 11868 edition 2 version 1
ICS:: 67.100.10

Тип поставки:

Full version150 €Read online 24h10 €Read online 72h15 €

Язык документа:

ruen

Срок поставки:2 раб. дн. (перевод)

Цена:150 €

Описание (английский):

ISO 11868|IDF 147:2007 specifies a method for the determination of the lactulose content of heated milk, skimmed, partially skimmed or whole milk, by high-performance liquid chromatography, in order to distinguish milk sterilized by ultra-heat treatment (UHT) from in-bottle sterilized milk. The method has been tested over a lactulose content range of 200 mg/l to 1 500 mg/l and is applicable to all heat-treated milks. The method described is to be used in cases of dispute.

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты ISO

Overview

ISO 11868:2007 (IDF 147:2007) defines a standardized laboratory method for the determination of lactulose in heat-treated milk using high-performance liquid chromatography (HPLC). The method applies to skimmed, partially skimmed and whole milks and is specifically intended to distinguish ultra‑heat treated (UHT) milk from in‑bottle sterilized milk. The procedure has been validated over a lactulose concentration range of 200 mg/L to 1 500 mg/L and is intended for use in formal dispute resolution and quality verification.

Key topics and technical requirements

Scope: Quantitative lactulose analysis for all heat‑treated milks (skimmed to whole).

Principle: Remove fat and protein, filter the sample, then measure lactulose in the filtrate by HPLC against calibrated standard samples.

Reagents: Analytical‑grade chemicals; a specified sample pretreatment solution containing zinc acetate dihydrate and phosphotungstic acid; lactulose standard (≥99 % purity); HPLC‑grade water as eluent.

Calibration: Use lactulose‑free pasteurized skim milk spiked to produce calibration levels (approx. 250, 500, 750 and 1 000 mg/L).

HPLC system: Column equivalent to Bio‑Rad HPX‑87 P (polystyrene divinylbenzene resin, sulfonic ion exchanger in lead form) with a de‑ashing precolumn; thermostatted column oven (~75 °C); pump flow typically 0.3–0.6 mL/min; refractive index (RI) detector with low noise and stable baseline.

Sample preparation: Controlled dilution, addition of pretreatment reagent, 1 hour standing, filtration through 0.45 μm membrane; degassing of filtrate before injection.

Performance and reporting: The standard includes instructions for calculation, repeatability/reproducibility assessment and the format of test reports. The method is designated for cases of dispute.

Applications and users

Quality control in dairy processing: Verification that pasteurization/sterilization processes meet product specifications and labeling claims (e.g., UHT vs in‑bottle sterilized).

Regulatory and enforcement laboratories: Official testing where provenance of heat treatment is contested.

Contract testing and certification bodies: Routine lactulose monitoring and comparative product testing.

Research laboratories: Studies of heat‑induced changes in milk chemistry and process optimization. Primary users: dairy chemists, analytical labs, food safety authorities, quality assurance managers, and forensic food analysts.

Related standards

ISO 707 | IDF 50 - Recommended sampling methods for milk (referenced for representative sampling).

ISO/IDF joint standards for milk analysis and heat‑treatment characterization.

Keywords: ISO 11868:2007, lactulose determination, HPLC, heat‑treated milk, UHT detection, dairy testing, milk analysis, refractive index detector.

ISO 11868:2007 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)

ISO 8689-1:2000

Water quality — Biological classification of rivers — Part 1: Guidance on the interpretation of biological qu…

ISO/ASTM51540-04(2012)

Standard Practice for Use of a Radiochromic Liquid Dosimetry System (Withdrawn 2020)

ISO/ASTM51204-04

Standard Practice for Dosimetry in Gamma Irradiation Facilities for Food Processing (Withdrawn 2013)

ISO/ASTM51431-05

Standard Practice for Dosimetry in Electron Beam and X-Ray (Bremsstrahlung) Irradiation Facilities for Food P…

ISO/ASTM52628-20e1

Standard Practice for Dosimetry in Radiation Processing

ISO/ASTM52921-13(2019)

Standard Terminology for Additive Manufacturing—Coordinate Systems and Test Methodologies

ISO/ASTMTR52917-EB

Additive Manufacturing — Round Robin Testing — General Guidelines