Overview
ISO 13365-2:2020 specifies an artificial perspiration extraction method for determining the aqueous-extractable content of leather preservatives - TCMTB, PCMC, OPP, and OIT - by liquid chromatography (HPLC/LC‑MS). The procedure assesses the portion of preservative agents that can be released from leather under simulated perspiration conditions, helping evaluate potential user exposure and product safety.
Key topics and technical requirements
- Scope: Quantification of TCMTB (2-(thiocyanomethylthio)-benzothiazole), PCMC (4-chloro-3-methylphenol), OPP (2-phenylphenol) and OIT (2-octylisothiazol-3(2H)-one) and their breakdown products using aqueous extraction and liquid chromatography.
- Extraction medium: Freshly prepared acid artificial perspiration solution (pH 5.5 ± 0.1) containing L-histidine·HCl, NaCl and NaH2PO4·2H2O.
- Sample extraction: Typically ~1 g leather with 10 ml artificial perspiration, extracted in a thermostatic shaker at 37 ± 2 °C for 24 ± 1 h.
- Analysis: Filtered extract analysed by HPLC with UV detection or LC‑MS. Calibration requires multiple concentrations and linear calibration curves.
- Calculations & reporting: Mass fraction expressed in mg/kg using the standard formula (concentration × extract volume × dilution factor ÷ sample mass). Results can be reported on a dry-matter basis per ISO 4684. Test report must reference ISO 13365-2 and include detection type and any deviations.
- Apparatus and reagents: Reversed-phase C8/C18 columns, HPLC or LC‑MS systems, specified reagent grades (HPLC/LC‑MS grade solvents, reference standards ≥97–99.7 %), membrane filters and controlled water quality (ISO 3696).
Practical applications
- Compliance and safety testing for leather products (footwear, garments, upholstery) to assess preservative release under simulated use.
- Quality-control testing in tanneries and leather finishing operations to monitor preservative levels.
- Exposure and risk assessment for product safety, regulatory submissions, and supplier verification.
- Analytical method for contract testing laboratories performing routine preservative screening.
Who should use this standard
- Leather technologists and chemists
- Quality assurance labs in tanneries and leather product manufacturers
- Third‑party testing laboratories and regulatory bodies
- Product safety and compliance teams evaluating chemical release from leather
Related standards
Keywords: ISO 13365-2, leather preservative testing, artificial perspiration extraction, TCMTB, PCMC, OPP, OIT, HPLC, LC‑MS, leather safety, preservative content.