ISO 13875:2005 PDF

Name: ISO 13875:2005 — Liquid milk — Determination of acid-soluble beta-lactoglobulin content — Reverse-phase HPLC method
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Liquid milk — Determination of acid-soluble beta-lactoglobulin content — Reverse-phase HPLC method

Название (английский):

ISO 13875:2005

Liquid milk — Determination of acid-soluble beta-lactoglobulin content — Reverse-phase HPLC method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 14
Дата публикации:: 5 января 2005 г.
Издание:: ISO IS 13875 edition 1 version 1
ICS:: 67.100.10

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Описание (английский):

ISO 13875|IDF 178:2005 specifies a method for the quantitative determination of the beta-lactoglobulin content, soluble at pH 4,6, in liquid milk. The method has been tested over a range between 0 mg and 3 500 mg of beta-lactoglobulin per litre of milk. It is suitable for distinguishing different categories of heat-treated liquid milk.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview - ISO 13875:2005 (IDF 178:2005)

ISO 13875:2005 specifies a reverse‑phase HPLC method for the quantitative determination of acid‑soluble β‑lactoglobulin (β‑LG) in liquid milk. The method measures the fraction of β‑LG that remains soluble after isoelectric precipitation at pH 4.6 and is validated over a range of 0 to 3 500 mg β‑LG per litre. It is designed to distinguish different categories of heat‑treated milks (raw, pasteurized, UHT, sterilized) and provides procedures for sample preparation, HPLC analysis, calibration and result expression.

Key topics and technical requirements

Principle: caseins and denatured whey proteins are precipitated at pH 4.6; the acid whey supernatant is analyzed by reverse‑phase HPLC to quantify soluble β‑LG.

Sample preparation: pH adjustment to 4.6, centrifugation (2 000 g), filtration and dilution into phosphate buffer; different dilution factors depending on milk heat treatment.

HPLC conditions: reverse‑phase column (PLRP‑S 150 × 4.6 mm, 5 or 8 µm or equivalent), gradient elution with HPLC‑grade water, acetonitrile and trifluoroacetic acid; UV detection at 205 nm or 280 nm; injection typically 20 µl; column temperature 40 °C.

Calibration: single‑point or multi‑point calibration against a standardized reference sample (freeze‑dried milk reference) and a pure β‑LG standard (A+B genetic variants). The standard details preparation and periodic standardization of reference material.

Reagents & safety: HPLC‑grade solvents and high‑purity trifluoroacetic acid; safety precautions for potentially hazardous elution solvents are recommended.

Apparatus: pH‑meter, centrifuge, microfiltration devices (0.22 µm), analytical balance and HPLC system with integrator/software for peak area measurement.

Quality and precision: the standard includes precision data and results from interlaboratory trials (see Annex A). It also defines reporting and expression of results.

Applications and who uses it

Dairy quality control labs monitoring protein denaturation or heat damage in liquid milk.

Milk processors comparing thermal processing regimes (pasteurization, UHT, sterilization).

Regulatory and food safety laboratories requiring standardized, traceable β‑LG measurements.

Research laboratories studying whey protein behavior, heat treatment effects and milk protein composition. Benefits include reliable differentiation of heat‑treated milk categories, traceable quantification of soluble β‑LG and compatibility with routine HPLC instrumentation.

Related standards

ISO 8968‑1 / IDF 20‑1 and ISO 8968‑2 / IDF 20‑2 (nitrogen/protein determination - Kjeldahl / block digestion) - normative references in ISO 13875.

ISO 707 (recommended sampling methods for milk) - sampling guidance referenced by the method.

Keywords: ISO 13875:2005, β‑lactoglobulin, beta‑lactoglobulin, reverse‑phase HPLC, acid‑soluble, liquid milk, dairy testing, heat‑treated milk, HPLC method.

ISO 13875:2005 PDF

Abstract

Похожие стандарты

Overview - ISO 13875:2005 (IDF 178:2005)

Key topics and technical requirements

Applications and who uses it

Related standards

Технические детали

SIST EN ISO 8968-1:2014

Milk and milk products - Determination of nitrogen content - Part 1: Kjeldahl principle and crude protein cal…

BS EN ISO 8968-2:2002

Milk. Determination of nitrogen content - Block-digestion method (Macro method)

BS ISO 13875:2005

Liquid milk. Determination of acid-soluble beta-lactoglobulin content. Reverse-phase HPLC method.

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)