ISO 14156:2025 PDF

Name: ISO 14156:2025 — Milk and milk products — Extraction methods for lipids and liposoluble compounds
SKU: ISO 14156:2025
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Milk and milk products — Extraction methods for lipids and liposoluble compounds

Название (английский):

ISO 14156:2025

Milk and milk products — Extraction methods for lipids and liposoluble compounds

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 6
Дата публикации:: 5 ноября 2025 г.
Издание:: ISO IS 14156 edition 2 version 1
ICS:: 67.100.01

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Описание (английский):

This document specifies methods for the extraction or separation of a representative part of the fat, containing lipids and liposoluble compounds, from milk and milk products. The method is applicable to the methods described in ISO 12078 | IDF 159, ISO 15884 | IDF 182, ISO 15885 | IDF 184 and ISO 18252 | IDF 200. NOTE Free fatty acids are not part of extracted fat as described in methods for the fat determination in milk, condensed milk, dried milk products, cream and fermented milk.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 14156:2025 (IDF 172:2025) specifies validated extraction and separation methods to obtain a representative fraction of fat - including lipids and liposoluble compounds - from milk and milk products. The standard supports reliable laboratory determination of fat content and composition by defining principles, required reagents and apparatus, sample preparation, and procedural safeguards. Note: free fatty acids are excluded from the extracted fat as described in these methods.

Key Topics

Principle: Extraction or physical separation of the lipid fraction from diverse dairy matrices (liquid milks, cream, butter, condensed and dried products, cheese) using solvent extraction or mechanical separation where appropriate.

Reagents: Analytical-grade solvents and reagents cited include ethanol, ammonia solution, diethyl ether, n-pentane, and anhydrous sodium sulfate. The standard emphasizes reagent quality and peroxide-free solvents where required.

Apparatus: Common laboratory equipment is specified, for example:

Soxhlet extraction apparatus and thimbles for exhaustive extraction
Separating funnels, water baths (various temperature ranges), rotary evaporator
Drying oven and filtration materials

Sample preparation: Conditioning and mixing instructions are provided for raw milk, homogenized milk, cream, evaporated and condensed milk, dried products and cheese to ensure a representative test portion.

Procedure highlights: Typical methods combine polar and non-polar solvents, phase separations and drying steps to isolate lipids and liposoluble constituents. For matrices rich in phospholipids (e.g., buttermilk), the choice of extraction influences fatty acid composition; the standard recommends tailored steps (and salt addition) to ensure phospholipid recovery.

Applications

ISO 14156:2025 is intended for use in analytical and quality control laboratories within the dairy industry and regulatory bodies. Practical applications include:

Routine fat determination and compositional analysis of milk and dairy products

Preparation of lipid extracts for further analyses (e.g., fatty acid profiling, lipid class analysis)

Method harmonization across laboratories for quality assurance and compliance with international testing protocols

Supporting product formulation, nutritional labelling and investigational studies where representative lipid extraction is required

Benefits: standardized procedures improve comparability of results, reduce analytical variability, and support robust QC programs.

Related Standards

ISO 12078 | IDF 159 - methods related to fat determination referenced by this document

ISO 15884 | IDF 182; ISO 15885 | IDF 184; ISO 18252 | IDF 200 - methods for which the extraction procedures are applicable

ISO 707 | IDF 50 - recommended sampling for milk and milk products

For implementation, laboratories should follow the reagent, apparatus and procedural guidance in ISO 14156:2025 and consult linked ISO/IDF methods for specific analytical endpoints.

ISO 14156:2025 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related Standards

Технические детали

ISO 12078:2006

Anhydrous milk fat — Determination of sterol composition by gas liquid chromatography (Reference method)

BS ISO 15884:2002

Milk fat. Preparation of fatty acid methyl esters.

BS ISO 15885:2002

Milk fat. Determination of fatty acid composition by gas-liquid chromatography.

SIST EN ISO 1825:2026

Rubber hoses and hose assemblies for aircraft ground fuelling and defuelling - Specification (ISO 1825:2026)

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)