ISO 15163:2012 PDF

Name: ISO 15163:2012 — Milk and milk products — Calf rennet and adult bovine rennet — Determination by chromatography of chymosin and bovine pepsin contents
SKU: ISO 15163:2012
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Milk and milk products — Calf rennet and adult bovine rennet — Determination by chromatography of chymosin and bovine pepsin contents

Название (английский):

ISO 15163:2012

Milk and milk products — Calf rennet and adult bovine rennet — Determination by chromatography of chymosin and bovine pepsin contents

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 19
Дата публикации:: 15 мая 2012 г.
Издание:: ISO IS 15163 edition 1 version 1
ICS:: 67.100.01

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Описание (английский):

ISO 15163:2012 specifies a reference method for the determination of the amounts of chymosin and bovine pepsin present in a test sample of calf rennet and adult bovine rennet. In addition, it can be used for mixtures of calf/bovine rennet with fermentation-produced bovine chymosin (FPC).

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты ISO

Overview

ISO 15163:2012 - "Milk and milk products - Calf rennet and adult bovine rennet - Determination by chromatography of chymosin and bovine pepsin contents" - is a reference analytical method published jointly by ISO and IDF. It specifies a chromatography-based procedure to quantify the amounts of chymosin and bovine pepsin in calf and adult bovine rennets, and can be applied to mixtures containing fermentation-produced bovine chymosin (FPC). Results are reported as percentage activities or as mg/L of active enzyme, supporting interpretation of milk‑clotting characteristics relevant to cheese production.

Key topics and technical requirements

Analytical principle: Desalting of rennet followed by separation of enzymes using anion-exchange chromatography; individual fractions are assayed for milk‑clotting activity using ISO 11815/IDF 157.

Manual and automated options: Procedures are provided for a manual Fractogel DEAE column setup and an automated set-up (e.g., Mono Q, FPLC/ÄKTA).

Reagents and buffers: Specified reagents include piperazine buffers, graded NaCl elution buffers, urea and ethanol options for column treatment; buffer preparation, degassing and preservation guidance are included.

Apparatus: Typical laboratory glassware, pH meter, peristaltic pump (manual), chromatography column or prepacked Mono Q (automated), and equipment for clotting-time determination.

Procedure elements: Sampling guidance (reference to ISO 707), desalting (dialysis or desalting columns), column preparation/regeneration, fraction collection, and clotting time determination.

Calculations and reporting: Conversion of clotting activity into International Milk‑Clotting Units (IMCU), expression of chymosin/pepsin as % of total activity or mg/L active enzyme; requirements for test reports.

Quality and performance: Method precision data, including repeatability and reproducibility from interlaboratory tests, are provided. The standard is a reference method - changes require validation and documentation in the test report.

Annexes: Informative annexes cover qualitative checks (double immunodiffusion) and interlaboratory test data.

Practical applications and users

Dairy laboratories performing quality control of liquid and powdered rennets.

Cheese manufacturers evaluating enzyme composition to predict curd formation, proteolysis and product suitability.

Rennet and enzyme suppliers verifying composition of calf/bovine rennet and FPC-containing blends.

Regulatory and standards bodies requiring traceable, reproducible quantification of milk-clotting enzymes.

Research groups studying the effects of enzyme composition on cheesemaking performance.

Related standards and references

ISO 11815 / IDF 157 - Determination of total milk‑clotting activity (used to assay separated fractions).

ISO 707 / IDF 50 - Recommended sampling methods for rennet products.

The standard also notes reference rennet powders with total activity fixed at 1 000 IMCU/g for calibration consistency.

Keywords: ISO 15163:2012, chymosin, bovine pepsin, calf rennet, chromatography, milk-clotting activity, FPC, anion exchange, dairy quality control.

ISO 15163:2012 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Practical applications and users

Related standards and references

Технические детали

ISO 11815:2007

Milk — Determination of total milk-clotting activity of bovine rennets

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)

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