ISO 16308:2014 PDF

Name: ISO 16308:2014 — Water quality — Determination of glyphosate and AMPA — Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection
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Water quality — Determination of glyphosate and AMPA — Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection

Название (английский):

ISO 16308:2014

Water quality — Determination of glyphosate and AMPA — Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 27
Дата публикации:: 8 сентября 2014 г.
Издание:: ISO IS 16308 edition 1 version 1
ICS:: 13.060.50

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Описание (английский):

ISO 16308:2014 specifies a method for the determination of dissolved fraction of glyphosate and its major metabolite, aminomethylphosphonic acid (AMPA), in drinking water, ground water, and surface water at concentrations of 0,03 µg/l to 1,5 µg/l. It does not apply to marine or salty water. This method can be applicable to other types of waters, provided the method is validated for each case.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview - ISO 16308:2014 (Water quality - Glyphosate and AMPA by HPLC–MS/MS)

ISO 16308:2014 specifies a validated analytical method for the determination of dissolved glyphosate and its main metabolite, aminomethylphosphonic acid (AMPA), in drinking water, groundwater and surface water. The standard describes an HPLC method coupled with tandem mass spectrometric detection (HPLC–ESI–MS/MS) with a practical working range of 0.03 µg/L to 1.5 µg/L (limits of quantification around 30 ng/L). It is not applicable to marine or salty water unless separately validated.

Key topics and technical requirements

Analytes addressed: glyphosate (N‑(phosphonomethyl)glycine) and AMPA; gluphosinate can be included if no matrix interference.

Analytical principle: pre‑column derivatization with 9‑fluorenylmethyl chloroformate (FMOC‑Cl) to reduce polarity and improve chromatographic retention and MS detection; analysis by HPLC coupled to triple‑quadrupole MS with electrospray ionization (ESI–MS/MS).

Sample preparation: filtration for dissolved fraction, optional liquid–liquid extraction and solid‑phase extraction (SPE) for pre‑concentration; direct injection is permitted if instrument sensitivity allows.

Calibration and quantification: matrix‑matched calibration and the use of isotopically labelled internal (surrogate) standards (13C,15N labelled glyphosate and AMPA) are specified to correct matrix effects.

Interferences & matrix effects: treatment of hard water (Ca/Mg) may require increased EDTA or acidification (Annex D). Free chlorine can oxidize analytes-sodium thiosulfate is required for dechlorination.

Quality control: blank monitoring, identification/confirmation criteria using MS/MS transitions, and performance data (Annex A) are included.

Instrumentation and reagents: guidance on HPLC columns (alkali‑stable C18 recommended), SPE cartridges, mobile phase buffers, and high‑purity solvents is provided.

Practical applications and users

ISO 16308 is intended for environmental and regulatory laboratories performing pesticide monitoring and water quality control. Typical applications include:

Regulatory compliance testing for drinking water and groundwater monitoring programs

Surface‑water monitoring for agricultural runoff and watershed contamination studies

Routine laboratory surveillance by water utilities and public health agencies

Research on pesticide fate, transport and degradation (AMPA formation)

Laboratories adopting this method benefit from sensitive, specific detection of glyphosate and AMPA with robust matrix compensation using isotopic surrogates.

Related standards (normative references)

ISO 3696 - Water for analytical laboratory use

ISO 5667‑3 - Water quality - Sampling: preservation and handling

ISO 8466‑1 - Calibration and evaluation of analytical methods (statistical evaluation)

Keywords: ISO 16308:2014, glyphosate, AMPA, HPLC–MS/MS, water quality, derivatization FMOC‑Cl, SPE, matrix‑matched calibration, drinking water testing.

ISO 16308:2014 PDF

Abstract

Похожие стандарты

Overview - ISO 16308:2014 (Water quality - Glyphosate and AMPA by HPLC–MS/MS)

Key topics and technical requirements

Practical applications and users

Related standards (normative references)

Технические детали

SIST ISO 16308:2018

Water quality - Determination of glyphosate and AMPA - Method using high performance liquid chromatography (H…

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

ISO 5667-3:2024

Water quality — Sampling — Part 3: Preservation and handling of water samples

BS ISO 8466-1:2021

Water quality. Calibration and evaluation of analytical methods - Linear calibration function