ISO 17180:2013 PDF

Name: ISO 17180:2013 — Animal feeding stuffs — Determination of lysine, methionine and threonine in commercial amino acid products and premixtures
SKU: ISO 17180:2013
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Animal feeding stuffs — Determination of lysine, methionine and threonine in commercial amino acid products and premixtures

Название (английский):

ISO 17180:2013

Animal feeding stuffs — Determination of lysine, methionine and threonine in commercial amino acid products and premixtures

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 11
Дата публикации:: 9 апреля 2013 г.
Издание:: ISO IS 17180 edition 1 version 1
ICS:: 65.120

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Описание (английский):

ISO 17180:2013 specifies a method for the quantitative determination of free (non-protein-bound) lysine, methionine, and threonine in commercial products and premixtures containing more than about 10 % mass fraction of the respective amino acid. It does not distinguish between d- and l-forms.

Abstract

Похожие стандарты

Другие стандарты ISO

Overview

ISO 17180:2013 specifies a validated analytical method for the quantitative determination of free (non‑protein‑bound) lysine, methionine and threonine in commercial amino‑acid products and premixtures used in animal feeding stuffs. The standard is applicable when the product contains about 10% or more mass fraction of the respective amino acid and does not distinguish between D‑ and L‑forms. The method uses an amino‑acid analyser or HPLC with a cation‑exchange column and post‑column derivatization for detection.

Key topics and technical requirements

Scope: Quantification of free lysine, methionine and threonine in commercial products and premixtures (> ~10%).

Principle: Acid extraction (dilute HCl), neutralization/dilution with sodium citrate buffer, addition of norleucine internal standard, chromatographic separation on a cation‑exchange column, and detection after post‑column reaction (ninhydrin colorimetry or OPA fluorescence).

Sample preparation: Grind to pass 0.25 mm, prepare two extracts per sample, and choose between dilution by weighing or volumetric dilution depending on sample type (pure amino acids vs. premixes).

Reagents & standards: Use high‑purity amino acid standards (≥99%) and norleucine internal standard (c = 2.5 mmol/L). Prepare calibration solutions by weight or volume dilution with sodium citrate buffer.

Chromatography & detection: Cation exchange resin, guard column, automated injection (10–100 µL typical), post‑column derivatization with ninhydrin (UV detection at 440/570 nm) or OPA (fluorescence 330/460 nm). Baseline separation of analyte peaks is required to avoid overlap.

Quality control & calibration: Inject calibration solution as QC after every four test samples; acceptable recovery range is 99–101%. The standard includes procedures for calculating results and addresses precision (repeatability and reproducibility).

Sample/storage notes: Filter extracts through 0.2 µm membrane; store below 5 °C (calibration solutions ≤1 week; sample extracts up to specified days).

Applications and who uses it

ISO 17180:2013 is used by:

Feed manufacturers and formulators for quality control of commercial amino‑acid products and premixtures.

Analytical and contract testing laboratories performing amino acid quantification for specification compliance.

Regulatory bodies and inspection laboratories validating label claims or investigating feed composition.

R&D teams optimizing feed formulations and ingredient quality.

Practical benefits include reproducible quantification of key essential amino acids (lysine, methionine, threonine), traceable calibration using an internal standard, and compatibility with common lab instrumentation (amino‑acid analyzers or HPLC).

ISO 17180:2013 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and who uses it

Related standards (if applicable)

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