ISO 22662:2024 PDF

Name: ISO 22662:2024 — Milk and milk products — Determination of lactose content by high-performance liquid chromatography (reference method)
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Milk and milk products — Determination of lactose content by high-performance liquid chromatography (reference method)

Название (английский):

ISO 22662:2024

Milk and milk products — Determination of lactose content by high-performance liquid chromatography (reference method)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 10
Дата публикации:: 7 марта 2024 г.
Издание:: ISO IS 22662 edition 2 version 1
ICS:: 67.100.01

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Описание (английский):

This document specifies a reference method for the determination of lactose content of raw milk, heat-treated milks, dried milk, dairy permeate, dairy permeate powder, and raw and pasteurized cream. The method does not apply to fermented milks and milks to which oligosaccharides have been added, nor to low-lactose or lactose-free milks.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 22662:2024 (IDF 198:2024) is the reference method for the determination of lactose content in a range of milk and milk products using high-performance liquid chromatography (HPLC). The scope covers raw milk, heat‑treated milks, dried milk, dairy permeate and permeate powder, and raw and pasteurized cream. The method is not applicable to fermented milks, milks with added oligosaccharides, or low‑lactose / lactose‑free products.

Key topics and technical requirements

Analytical principle: Addition of an internal standard (d(+)-melezitose) to sample and standards, protein/fat precipitation with Biggs‑Szijarto reagent, double filtration (paper then 0.45 µm nylon), separation on a lead‑form cation exchange column and detection by differential refractometer or other suitable detector. Mobile phase: degassed HPLC‑grade water.

Reagents: degassed HPLC water (ISO 3696 grade 1), d(+)-melezitose hydrate (≈50 mg/ml solution), α‑lactose monohydrate (dried at 70 °C for 4 h), and Biggs‑Szijarto solution (zinc acetate + phosphotungstic acid + acetic acid).

Apparatus: HPLC ion‑exchange column (300 mm × 7.8 mm, lead form), guard column and micro‑guard holder, column heater maintained at 85 °C ± 1 °C, HPLC pump/autosampler, differential refractometer detector, 0.45 µm syringe or in‑line filters, precision balance, water purification and solvent filtration units.

Procedure highlights: sample warming and homogenization, internal standard addition, precipitation and removal of fats/proteins, preparation of filtrate, HPLC setup and chromatographic conditions, calculation and expression of lactose mass fraction.

Quality and performance: the document includes precision data (repeatability, reproducibility) from interlaboratory testing and prescribes reporting requirements.

Practical applications

Regulatory and official control laboratories validating lactose content claims.

Dairy industry quality control for raw, processed and powdered products.

Contract testing laboratories and accreditation bodies requiring a standardized reference method.

Research laboratories conducting comparative studies of carbohydrate composition in milk matrices.

ISO 22662:2024 provides a robust, validated HPLC protocol for accurate quantification of lactose where precise measurement is required for compliance, lab accreditation, nutritional labelling, product development, and quality assurance.

Related standards

ISO 707 | IDF 50 - recommended sampling of milk and milk products

ISO 3696 - water for analytical laboratory use

ISO 648 - laboratory glassware (single‑volume pipettes)

Keywords: ISO 22662:2024, lactose determination, HPLC lactose method, milk and milk products, dairy permeate, reference method, lactose content analysis.

ISO 22662:2024 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Practical applications

Who should use this standard

Related standards

Технические детали

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

SIST EN ISO 707:2009

Milk and milk products - Guidance on sampling (ISO 707:2008)

ISO 6487:2015/Amd 1:2017

Road vehicles — Measurement techniques in impact tests — Instrumentation — Amendment 1