Name: ISO 23719:2025 — Cereals and cereal products — Determination of 17 mycotoxins by ultra-high-performance liquid chromatography and tandem mass spectrometry method (UHPLC-MS/MS)
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Abstract

Overview

ISO 23719:2025 specifies a quantitative UHPLC‑MS/MS method for the determination of 17 mycotoxins in cereals and cereal products. The standard covers common commodities such as wheat, maize, husked rice and rice products, and uses an ultra‑high‑performance liquid chromatography tandem mass spectrometry (UHPLC‑MS/MS) workflow with isotopic dilution. Target analytes include aflatoxins (B1, B2, G1, G2), deoxynivalenol (and derivatives), nivalenol, zearalenone, ochratoxin A, fumonisins (B1, B2, B3), T‑2 and HT‑2 toxins, and sterigmatocystin. ISO 23719:2025 does not apply to foods for infants and young children and can be extended to other matrices (e.g., nuts) only after matrix‑specific validation.

Key Topics

Analytical principle: Extraction with acetonitrile–water–acetic acid (70:29:1, v/v/v), low‑temperature centrifugation, filtration, addition of isotope‑labelled internal standards, and analysis by UHPLC‑MS/MS in multiple reaction monitoring (MRM) mode.
Isotopic dilution: Quantification via 13C‑labelled internal standards (ISTDs) for robust accuracy and compensation for matrix effects.
Calibration and validation: Defined calibration ranges and validated ranges from an interlaboratory study (see Annex A). Calibration solutions and mixed stock/ISTD preparations are specified.
Instrument requirements: UHPLC capable of binary gradients, column retention performance, column oven, and a tandem mass spectrometer with electrospray ionization (ESI).
Safety and quality: Mycotoxin hazard warnings, reagent quality (LC‑grade solvents), sample preparation controls, and precision metrics (repeatability and reproducibility).
Supporting information: Annexes provide ISTD lists, example LC‑MS/MS conditions, sample chromatograms, and interlaboratory results.

Applications

Food testing laboratories implementing routine mycotoxin surveillance in cereals and cereal products.
Regulatory agencies performing compliance testing and exposure assessment for aflatoxins, DON, fumonisins, ZEN, OTA and others.
Quality assurance teams in milling, grain trading and cereal processing industries for internal control and supplier verification.
Method developers and validation labs needing a robust UHPLC‑MS/MS protocol and interlaboratory performance benchmarks.

Using ISO 23719:2025 helps labs achieve traceable, harmonized results for multiple mycotoxins with high sensitivity and specificity, improving food safety decision‑making and trade compliance.

Related standards

ISO 3696 - Water for analytical laboratory use (normative reference in ISO 23719:2025)
Prepared by ISO/TC 34/SC 4 (Cereals and pulses); see full annexes in ISO 23719:2025 for calibration ranges, ISTD details and example conditions.

ISO 23719:2025 PDF

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Abstract

Overview

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Applications

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