ISO 23753-1:2019 PDF
Soil quality — Determination of dehydrogenases activity in soils — Part 1: Method using triphenyltetrazolium chloride (TTC)
Soil quality — Determination of dehydrogenases activity in soils — Part 1: Method using triphenyltetrazolium chloride (TTC)
- Статус документа:
- Действующий
- Формат:
- Электронный (PDF)
- Количество страниц:
- 9
- Дата публикации:
- 15 февраля 2019 г.
- Издание:
- ISO IS 23753 edition 2 version 1
- ICS:
- 13.080.30
This document specifies a method for determining the activity of dehydrogenases enzymes in soil using 2,3,5-triphenyltetrazolium chloride (TTC).
Abstract
Overview
ISO 23753-1:2019 - "Soil quality - Determination of dehydrogenases activity in soils - Part 1: Method using triphenyltetrazolium chloride (TTC)" specifies a standardized enzymatic assay to quantify soil microbial dehydrogenase activity. The method adds TTC to soil, incubates to form triphenylformazan (TPF), extracts the TPF and measures it by spectrophotometry at 485 nm. Dehydrogenase activity (DHA) is a widely used indicator of viable microbial oxidative activity and overall soil biological functioning.
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