ISO 24583:2022 - Overview
ISO 24583:2022 specifies general requirements and performance criteria for purity determination of organic compounds used in foods and food products by solution‑state proton quantitative nuclear magnetic resonance (1H qNMR) using an internal standard method. The standard is applicable to bioactive compounds in functional foods, natural toxins, food additives and pesticides, and is intended for users pursuing metrological traceability of measurement results.
Key topics and technical requirements
The standard defines a validated qNMR workflow and addresses technical topics essential for reliable purity determination:
- Principles of 1H qNMR
- Chemical shift interpretation, spin–spin coupling and the proportionality between integrated peak area and number of protons.
- qNMR procedure and study design
- Predetermined workflows covering sample solution preparation, acquisition parameters and data processing that are optimized and validated for a specific analyte.
- Technical measurement requirements
- Acquisition parameters (spectral width, repetition time, number of transients, flip angle), shimming, receiver gain, dummy scans and FID handling.
- Data processing and purity calculation
- Phase and baseline correction, zero‑filling, line‑broadening considerations, integration and calculation of sample purity using an internal standard.
- Uncertainty and traceability
- Evaluation of measurement uncertainty and establishment of metrological traceability (use of certified reference materials to link results to the SI).
- Validation and instrument qualification
- Validation of qNMR procedures and NMR instrument qualification to ensure repeatability and performance.
- Supporting information
- Informative annexes on weighing (Annex A), recommended internal standards and solvents (Annex B), and uncertainty determination (Annex C).
Note: the document states there are no normative references; it does, however, reference metrology guidance such as ISO/IEC Guide 99 and EURAMET Calibration Guide No.18 within definitions and background.
Practical applications and users
ISO 24583:2022 is designed for laboratories and organizations involved in food analysis and metrology:
- Food testing and regulatory laboratories performing purity checks on additives, contaminants and bioactives.
- National metrology institutes and producers of certified reference materials (CRMs) seeking SI traceability for purity values.
- Food manufacturers and ingredient suppliers requiring validated methods to certify raw materials.
- Researchers developing qNMR methods for food authenticity, contamination screening and standard characterization.
Benefits include a standardized approach to 1H qNMR purity assays, enhanced comparability of results, and a clear route to metrological traceability.
Related standards and resources
- ISO/IEC Guide 99 (VIM) - for metrological terms and traceability concepts (referenced in the document).
- EURAMET Calibration Guide No.18 - referenced for weighing/measurement considerations.
Keywords: ISO 24583:2022, 1H qNMR, quantitative NMR, purity determination, internal standard, metrological traceability, food additives, natural toxins, bioactive compounds.