ISO 29822-2:2026 PDF

Name: ISO 29822-2:2026 — Vegetable fats and oils — Determination of the content and relative amounts of 1,2- and 1,3-diacylglycerols — Part 2: Isolation by solid phase extraction (SPE)
SKU: ISO 29822-2:2026
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Vegetable fats and oils — Determination of the content and relative amounts of 1,2- and 1,3-diacylglycerols — Part 2: Isolation by solid phase extraction (SPE)

Название (английский):

ISO 29822-2:2026

Vegetable fats and oils — Determination of the content and relative amounts of 1,2- and 1,3-diacylglycerols — Part 2: Isolation by solid phase extraction (SPE)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 10
Дата публикации:: 19 марта 2026 г.
Издание:: ISO IS 29822 edition 1 version 1
ICS:: 67.200.10

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Описание (английский):

This document specifies the determination of the absolute value of diacylglycerols as well as the degree of isomerization of diacylglycerols in vegetable fats and oils. 1,2-diacylglycerols are transformed to the more stable 1,3-isomers during storage or due to acidic catalysed reaction. NOTE Results obtained using this document have not been compared with results obtained using ISO 29822.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 29822-2:2026 specifies the method for determining the absolute content and relative amounts of 1,2- and 1,3-diacylglycerols in vegetable fats and oils using solid phase extraction (SPE). Diacylglycerols (DAGs) are important indicators of quality and authenticity in oils, as the 1,2-DAG isomer can isomerize to the more stable 1,3 form during storage or under acidic conditions. This international standard provides a reliable approach for isolating DAG fractions and establishing their degree of isomerization, supporting consistent quality assessment and authenticity verification of edible oils.

The method outlined in this standard offers a streamlined SPE-based approach, which requires less solvent and preparation compared to traditional silica gel chromatography. This enhances laboratory efficiency for routine analysis, quality control, and research applications involving vegetable oils.

Key Topics

Measurement of 1,2- and 1,3-diacylglycerols: Defines procedures for quantifying these isomers as indicators of oil quality and processing history.

Solid phase extraction (SPE): Utilizes a diol phase SPE column to isolate DAGs, reducing solvent use and minimizing isomerization artifacts common with silica gel columns.

Gas chromatography analysis: Following SPE and silylation, capillary gas chromatography is employed to separate and quantify DAG isomers, ensuring high specificity and sensitivity.

Calculation of content and isomer ratios: Mass fractions of DAGs are determined, supporting consistent reporting according to ISO requirements.

Precision and repeatability: Establishes criteria for repeatability and reproducibility based on interlaboratory studies, ensuring confidence in results.

Test reporting: Details the necessary components of test reports, promoting transparency and traceability in oil analysis.

Selection of internal standards: Recommends specific internal standards for accurate quantitation, especially for oils high in palmitic acid.

Applications

ISO 29822-2:2026 is widely applicable in industries and laboratories concerned with the quality and authenticity of vegetable fats and oils. Key areas of use include:

Quality control: Routine testing in oil production facilities to ensure product integrity and compliance with quality standards.

Authenticity assessment: Differentiation between genuine and adulterated vegetable oils by analyzing isomerization patterns.

Shelf-life and storage studies: Monitoring the transformation of 1,2-DAG to 1,3-DAG during storage, which is relevant for product stability and labeling.

Research and development: Used in oil chemistry research for method development, process optimization, and formulation studies.

Regulatory compliance: Supports food industry compliance with national and international oil standards by providing an ISO-validated method.

Laboratory accreditation: The method aids laboratories seeking accreditation by supporting standardized, reproducible analytical protocols.

Related Standards

ISO 29822: Vegetable fats and oils - Isomeric diacylglycerols - Determination of relative amounts of 1,2- and 1,3-diacylglycerols.

ISO 5555: Animal and vegetable fats and oils - Sampling (provides guidance for representative sample collection).

ISO 661: Animal and vegetable fats and oils - Preparation of test sample (for sample handling and preparation).

ISO 5725-1 & ISO 5725-2: Accuracy (trueness and precision) of measurement methods and results - General principles, and determination of repeatability and reproducibility.

ISO 29822-2:2026 delivers a scientifically robust and practical approach for the reliable determination of diacylglycerol isomers in vegetable fats and oils, making it an essential tool for laboratories and producers aiming to meet international quality benchmarks in the edible oils industry.

ISO 29822-2:2026 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related Standards

Технические детали

BS EN ISO 29822:2014

Vegetable fats and oils. Isomeric diacylglycerols. Determination of relative amounts of 1,2- and 1,3-diacylgl…

ISO 5555:2001/Amd 1:2014

Animal and vegetable fats and oils — Sampling — Amendment 1: Flexitanks

ISO 6614:1994/Amd 1:2019

Petroleum products — Determination of water separability of petroleum oils and synthetic fluids — Amendment 1

SIST ISO 5725-1:2024

Accuracy (trueness and precision) of measurement methods and results - Part 1: General principles and definit…

SIST ISO 5725-2:2020

Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determina…