ISO 4134:2021 PDF

Name: ISO 4134:2021 — Meat and meat products — Determination of L-(+)-glutamic acid content — Reference method
SKU: ISO 4134:2021
Price: 25 EUR
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Meat and meat products — Determination of L-(+)-glutamic acid content — Reference method

Название (английский):

ISO 4134:2021

Meat and meat products — Determination of L-(+)-glutamic acid content — Reference method

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 15
Дата публикации:: 23 июля 2021 г.
Издание:: ISO IS 4134 edition 3 version 1
ICS:: 67.120.10

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Описание (английский):

This document specifies the spectrophotometer method and the light absorption microplate reader method for the determination of the free L-(+)-glutamic acid content of meat and meat products. This document is applicable to meat and meat products, including livestock and poultry products.

Abstract

Overview

Key topics and requirements

Scope and applicability: Free L-(+)-glutamic acid in fresh and processed meat and poultry products.
Principle: Extraction of free glutamic acid with perchloric acid, pH adjustment (to alkaline), enzymatic reduction of NAD by glutamate dehydrogenase, NADH-driven reduction of iodonitrotetrazolium chloride via diaphorase to form a formazan dye measured at 490 nm.
Two validated methods:
- Spectrophotometer method (detailed reagents, apparatus, extraction and measurement steps).
- Light absorption microplate reader method (higher throughput option using microplates).
Reagents and conditions: Includes dilute perchloric acid extraction (c = 1.0 mol/L), triethanolamine phosphate buffer (pH 8.6), KOH solutions for neutralization, and defined enzyme/NAD mixtures.
Analytical performance: Includes instructions for calibration (standard curves and linear regression), calculation of L-(+)-glutamic acid concentration, and documented precision metrics - repeatability, reproducibility, interlaboratory test results and detection limit.
Sampling & sample prep: Sampling is not prescriptive but recommends representative sampling (see CAC/GL 50-2004), homogenization, temperature control and storage instructions to avoid composition changes.
Reporting and safety: Specifies minimum reporting content for test reports and includes an informative annex on safety practices when handling strong acids and reagents.

Applications

Quality control in meat processing (flavour profiling, glutamate content monitoring).
Regulatory and compliance testing where free glutamate must be quantified.
Food research and product development investigating umami contributors or ingredient effects.
High-throughput laboratories can adopt the microplate reader method for increased sample throughput while maintaining reference-method traceability.

Who should use this standard

Food testing laboratories, meat processors, regulatory agencies, contract testing providers, and researchers requiring a validated reference method for quantifying free L-(+)-glutamic acid in meat and poultry products.

Related standards

Normative references cited include ISO 648, ISO 1042, ISO 1442 (moisture reference), ISO 3696 (water for analytical use) and ISO 8655‑2 (piston pipettes). These support laboratory equipment, sampling and quality controls referenced in ISO 4134:2021.

Технические детали

Технический комитет: ISO/TC 34/SC 6 - Meat, poultry, fish, eggs and their products
SKU: ISO 4134:2021

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ISO 4134:2021 PDF

Abstract

Overview

Похожие стандарты

Key topics and requirements

Applications

Who should use this standard

Related standards

Технические детали

ISO 6487:2015/Amd 1:2017

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BS EN ISO 10427-1:2024

Oil and gas industries including lower carbon energy. Equipment for well cementing. Casing bow-spring central…

SIST ISO 1442:2024

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SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

SIST EN ISO 8655-2:2022

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