ISO 4274:1977 PDF

Applicable to products having contents of equal to or more than 0.05 % (m/m) (with flame atomic absorption) or 0.1 % (m/m) (with fotometric absorption). Covers the formation of a complex between biuret and copper(II) sulphate in alkaline medium. Fixation of this complex on an anion exchange resin. Elution of Cu first with a potassium nitrate solution and then with a nitric acid solution. Flame atomic absorption: aspiration of the solution into an acetylene-air flame and measurement of the absorption of the 325-nm line emitted by a copper hollow-cathode lamp; or by photometric absorption: formation of a coloured complex between Cu and zinc dibenzyldithiocarbamate, extraction with carbon tetrachloride and measurement at a wavelength of about 435 nm. Has also been approved by the IUPAC.