ISO 6870:2002 PDF

Name: ISO 6870:2002 — Animal feeding stuffs — Qualitative determination of zearalenone
SKU: ISO 6870:2002
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Animal feeding stuffs — Qualitative determination of zearalenone

Название (английский):

ISO 6870:2002

Animal feeding stuffs — Qualitative determination of zearalenone

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 8
Дата публикации:: 30 мая 2002 г.
Издание:: ISO IS 6870 edition 2 version 1
ICS:: 65.120

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Описание (английский):

This International Standard specifies a qualitative method for the determination of zearalenone in animal feeding stuffs and, in particular, in maize. This method is for screening purposes only. The limit of determination of zearalenone is approximately 50 μg/kg . NOTE Although sorghum gives interfering fluorescent spots identical to those of zearalenone, the method is still applicable to this feed because Rf the values are different after development of the chromatogram in the second direction. These spots are not developed by the specified confirmation technique.

Abstract

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Упомянутые в описании и другие стандарты ISO

Overview

ISO 6870:2002 specifies a qualitative screening method for the detection of zearalenone in animal feeding stuffs, with particular emphasis on maize. The method is intended for screening (not quantitative compliance testing) and has a reported limit of determination of approximately 50 µg/kg. The procedure uses two-dimensional thin‑layer chromatography (2D‑TLC) with UV fluorescence detection and a confirmatory spray (bis‑diazotized benzidine).

Key topics and technical requirements

Scope: Qualitative screening of zearalenone in animal feedstuffs (maize emphasized). Sorghum interference is addressed by 2D chromatographic separation and the specified confirmation technique.

Sample preparation: Grind to 1 mm, 50 g test portion, extract with acetonitrile and KCl solution, defat with isooctane, concentrate.

Purification: Treat extract with lead acetate in the presence of diatomaceous earth (Celite), then extract into chloroform and evaporate.

Chromatography: Dissolve residue in benzene/acetonitrile and perform two‑direction TLC using specified solvent systems; apply standard spots alongside sample for comparison.

Detection & confirmation:

Visual comparison of UV‑induced fluorescence (short‑wave UV) or measurement by fluorodensitometer (preferred where available).
Confirm identity by spraying with bis‑diazotized benzidine reagent (zearalenone produces a brick‑red spot).

Performance & precision: An interlaboratory study (Annex A) summarizes repeatability and reproducibility data for maize matrices; the method is explicitly for screening and precision values are matrix/concentration dependent.

Safety: The method uses hazardous solvents and reagents (chloroform, benzene, benzidine). Use fume hoods, appropriate PPE, and follow laboratory safety rules.

Practical applications

Rapid screening of feed materials (especially maize) for zearalenone contamination before more definitive testing.

On‑site or laboratory feed safety monitoring programs to flag suspect batches for follow‑up quantitative analysis.

Pre‑screening in feed manufacturing, grain trading, quality control, and regulatory surveillance to prioritize samples for confirmatory methods (e.g., LC‑MS/MS).

Useful where a cost‑effective, relatively simple TLC‑based screening is required.

Related standards

Prepared by ISO/TC 34 / SC 10 (Animal feeding stuffs).

Precision evaluation in the standard references ISO 5725 methodology for repeatability and reproducibility.

Keywords: ISO 6870:2002, zearalenone, animal feeding stuffs, maize, qualitative screening, thin‑layer chromatography, 2D‑TLC, bis‑diazotized benzidine, feed safety.

ISO 6870:2002 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Practical applications

Who should use this standard

Related standards

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