ISO 8128-1:1993 PDF

Name: ISO 8128-1:1993 — Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content — Part 1: Method using high-performance liquid chromatography
SKU: ISO 8128-1:1993
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Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content — Part 1: Method using high-performance liquid chromatography

Название (английский):

ISO 8128-1:1993

Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content — Part 1: Method using high-performance liquid chromatography

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 4
Дата публикации:: 24 июня 1993 г.
Издание:: ISO IS 8128 edition 1 version 1
ICS:: 67.160.20

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Описание (английский):

The principle of the method specified is extraction of patulin from a test portion using ethyl acetate followed by partitioning of the extract with aqueous sodium carbonate solution, qualitative and quantitative determination of the patulin content by means of high-performance liquid chromatography using an ultraviolet detector. The limit of detection is 10 µg/l, based on 5 ml of ready-to-drink apple juice.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO 8128-1:1993 is an international standard developed by the International Organization for Standardization (ISO). This standard specifies a method for the determination of patulin content in apple juice, apple juice concentrates, and drinks containing apple juice, using high-performance liquid chromatography (HPLC). Patulin is a mycotoxin commonly found in products derived from apples, and its accurate quantification is essential for food safety and regulatory compliance. The method outlined is recognized for its sensitivity, with a detection limit of 10 µg/l based on a 5 ml sample of ready-to-drink apple juice.

Key Topics

Sample Preparation: Includes detailed procedures for extracting patulin from test portions using ethyl acetate, followed by partitioning with aqueous sodium carbonate.

Analytical Method: Utilizes high-performance liquid chromatography with an ultraviolet (UV) detector for both qualitative and quantitative measurements.

Reagents and Apparatus: Specifies the use of high-grade ethyl acetate, acetonitrile, sodium carbonate, and related laboratory instruments, including a UV detector calibrated at 276 nm and a reverse-phase HPLC column.

Calibration and Calculation: Describes the preparation of standard solutions, calibration curves, and the calculation method for determining the patulin content in micrograms per litre.

Precision and Reproducibility: Outlines repeatability and reproducibility statistics in accordance with ISO 5725, ensuring consistent and reliable results across laboratories.

Reporting Requirements: Highlights essential elements for the test report, including the procedure used, test results, and all relevant operational details.

Applications

ISO 8128-1:1993 is widely applicable in several sectors, including:

Food Safety Laboratories: Supports regulatory and quality assurance testing to ensure compliance with maximum patulin limits in apple-based beverages and concentrates.

Juice and Beverage Industry: Assists manufacturers in routine quality control, allowing them to monitor and manage patulin levels to meet international food safety standards.

Government and Regulatory Agencies: Provides a reference method for surveillance, enforcement, and harmonization of food safety standards related to mycotoxin contamination.

Research Institutions: Facilitates studies on food contaminants and advances research in food chemistry by providing a validated and reproducible analytical technique.

Related Standards

ISO 8128-2:1993 - "Apple juice, apple juice concentrates and drinks containing apple juice - Determination of patulin content - Part 2: Method using thin-layer chromatography": Offers an alternative analytical procedure for patulin determination.

ISO 5725:1986 - "Precision of test methods – Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests": Provides the statistical foundation for evaluating measurement consistency in ISO 8128-1.

AOAC Official Methods and international literature on analytical methods for patulin and mycotoxin determination.

Practical Value

Implementing ISO 8128-1:1993 ensures:

Reliable Detection: High sensitivity and specificity for patulin quantification, crucial for public health protection and regulatory compliance.

Standardization: Harmonized methodology improves comparability of results across industries and borders.

Consumer Confidence: Assures the safety and quality of apple juice and related products, supporting market access and brand reputation.

By adhering to this ISO standard, laboratories and producers can confidently manage patulin risks and deliver safer, high-quality apple-based beverages worldwide.

ISO 8128-1:1993 PDF

Abstract

Похожие стандарты

Overview

Key Topics

Applications

Related Standards

Practical Value

Технические детали

ISO 8128-2:1993

Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content —…

ISO 5725:1986

Precision of test methods — Determination of repeatability and reproducibility for a standard test method by…

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Accuracy (trueness and precision) of measurement methods and results — Part 6: Use in practice of accuracy va…

SIST ISO 8128-1:2000

Apple juice, apple juice concentrates and drinks containing apple juice -- Determination of patulin content -…