ISO/TS 12788:2022 PDF

Name: ISO/TS 12788:2022 — Rapeseed — Determination of glucosinolate content — Spectrometric method for total glucosinolates by glucose release
SKU: ISO/TS 12788:2022
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Rapeseed — Determination of glucosinolate content — Spectrometric method for total glucosinolates by glucose release

Название (английский):

ISO/TS 12788:2022

Rapeseed — Determination of glucosinolate content — Spectrometric method for total glucosinolates by glucose release

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 20
Дата публикации:: 18 мая 2022 г.
Издание:: ISO TS 12788 edition 1 version 1
ICS:: 67.200.20

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Срок поставки:4 раб. дн. (перевод)

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Описание (английский):

This document specifies a method for the determination of the content of the total glucosinolates in rapeseeds (colza) using visible spectrometry to determine the glucose released from glucosinolates by hydrolysis.

Abstract

Overview

Key topics and technical requirements

Scope and principle: Total glucosinolates are quantified by hydrolysing glucosinolates with myrosinase, collecting the released glucose and determining glucose concentration enzymatically. Results are expressed as micromoles of glucose per gram of seed.
Sample preparation and sampling: Requirements reference standard oilseed laboratory practices for reduction to test sample, moisture determination and grinding to a laboratory sample (see ISO 664, ISO 665).
Reagents and buffers: The specification lists required reagents (e.g., phenol tungstate reagent, sodium phosphate buffer, myrosinase solution) and guidance on preparing calibration glucose standards.
Glucose assays: Two validated enzymatic assay routes are provided:
- Glucose oxidase / peroxidase colorimetric assay (visible spectrometry)
- Glucose hexokinase / glucose-6-phosphate dehydrogenase assay (spectrophotometric NADPH measurement)
Procedure steps: Extraction of glucosinolates, enzymatic hydrolysis, glucose collection (column chromatography steps described), glucose determination, and calculation of total glucosinolate content.
Quality and precision: The document includes guidance on calculation, expression of results, repeatability/reproducibility considerations and reports results of an interlaboratory study (Annex C). Annexes also cover myrosinase extraction from Sinapis alba and recovery determination.

Applications and users

ISO/TS 12788:2022 is practical for:

Seed testing and quality control laboratories measuring glucosinolate levels in rapeseed/canola
Oilseed breeders and research labs screening lines for low-glucosinolate traits
Commercial grading and acceptance testing where rapid, non-chromatographic estimation is required
Feed and food safety analysts monitoring antinutritional and flavor-related glucosinolate content

The method is useful when chromatographic equipment is unavailable or when a rapid total-glucosinolate screening is needed.

Related standards

ISO 9167 - Reference chromatographic methods for glucosinolates
ISO 664 - Oilseeds - Reduction of laboratory sample to test sample
ISO 665 - Oilseeds - Determination of moisture and volatile matter content
ISO 3696 - Water for analytical laboratory use

Keywords: ISO/TS 12788:2022, glucosinolate content, rapeseed, spectrometric method, glucose release, myrosinase, glucose assay, canola, oilseeds, analytical method.

Технические детали

Технический комитет: ISO/TC 34/SC 2 - Oleaginous seeds and fruits and oilseed meals
SKU: ISO/TS 12788:2022

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ISO/TS 12788:2022 PDF

Abstract

Overview

Похожие стандарты

Key topics and technical requirements

Applications and users

Related standards

Технические детали

SIST EN ISO 9167:2019

Rapeseed and rapeseed meals - Determination of glucosinolates content - Method using high-performance liquid…

SIST EN ISO 6647-1:2020

Rice - Determination of amylose content - Part 1: Spectrophotometric method with a defatting procedure by met…

SIST EN ISO 665:2020

Oilseeds - Determination of moisture and volatile matter content (ISO 665:2020)

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)