ISO/TS 18827:2017 PDF

Name: ISO/TS 18827:2017 — Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials
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Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials

Название (английский):

ISO/TS 18827:2017

Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 20
Дата публикации:: 23 мая 2017 г.
Издание:: ISO TS 18827 edition 1 version 1
ICS:: 07.030

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Описание (английский):

ISO/TS 18827:2017 provides a procedure for the detection of ROS (OH, O2-, 1O2) generated by metal oxide nanomaterials in aqueous solution with a reactive oxygen species-specific spin trapping agent using ESR, but excludes ESR procedures that do not use a spin trapping agent.

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты ISO

Overview

ISO/TS 18827:2017 - Nanotechnologies - Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials - specifies a standardized, spin-trapping ESR procedure to detect and quantify ROS produced by metal oxide nanomaterials in aqueous, cell-free conditions. The technical specification targets detection of the biologically relevant ROS: hydroxyl radical (•OH), superoxide anion (O2•–) and singlet oxygen (1O2) using species-specific spin traps and excludes ESR methods that do not use spin trapping agents.

Key topics and requirements

Purpose: Provide a reproducible ESR-based method to assess ROS generation potential of metal oxide nanomaterials as part of nanotoxicity evaluation and physico‑chemical characterisation.

Target ROS and spin traps:

DMPO for hydroxyl radical (DMPO/•OH adduct)
BMPO for superoxide (BMPO/OOH adduct)
TPC for singlet oxygen (TPC/1O2 adduct)

Positive controls and validation: describes use of established radical-generating systems (e.g., Fenton reaction, hypoxanthine–xanthine oxidase, rose bengal photosensitization) and zero-baseline controls to demonstrate assay suitability.

Procedure elements: preparation of nanomaterial suspensions, reagents and spin trap stock solutions, reaction conditions, sample injection and ESR measurement of spin adduct spectra, and calculation/standardization steps.

Interferences and limitations: addresses sampling, timing, potential assay interferences, and the importance of controlling physico-chemical parameters (pH, light, aggregation) that influence ROS formation.

Output: ESR spectra (spin adduct fingerprints) enabling detection and semi-quantitative assessment of ROS generation.

Applications and who should use it

Researchers and laboratories characterising metal oxide nanomaterials (e.g., TiO2, ZnO) for oxidative potential.

Nanotoxicologists and risk assessors evaluating ROS-mediated cytotoxicity and mechanistic hazard data.

Product developers in biomedical devices, cosmetics, coatings, pigments, and environmental nanomaterials seeking early-stage screening of oxidative reactivity.

Quality control and regulatory testing labs requiring standardized ESR spin‑trapping methods to support safety dossiers and comparability studies.

Practical value: ISO/TS 18827:2017 provides a reproducible, cell‑free screen to predict oxidative stress potential, support hazard identification, and inform further in vitro or in vivo testing strategies.

Related standards

ISO/TS 80004‑1:2015 (nanotechnology vocabulary) - terminology referenced in the specification.

ISO/TS 10993‑5:2009 and ISO/TS 10993‑12:2012 - referenced for test sample and control definitions used in biological testing contexts.

Keywords: ISO/TS 18827:2017, ESR, spin trapping, ROS, nanotechnologies, metal oxide nanomaterials, DMPO, BMPO, TPC, nanotoxicity, reactive oxygen species.

ISO/TS 18827:2017 PDF

Abstract

Похожие стандарты

Overview

Key topics and requirements

Applications and who should use it

Related standards

Технические детали

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