ISO/TS 22082:2020 PDF

Name: ISO/TS 22082:2020 — Nanotechnologies — Assessment of nanomaterial toxicity using dechorionated zebrafish embryo
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Nanotechnologies — Assessment of nanomaterial toxicity using dechorionated zebrafish embryo

Название (английский):

ISO/TS 22082:2020

Nanotechnologies — Assessment of nanomaterial toxicity using dechorionated zebrafish embryo

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 14
Дата публикации:: 6 мая 2020 г.
Издание:: ISO TS 22082 edition 1 version 1
ICS:: 07.120

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Описание (английский):

This document specifies a method for rapidly assessing nanomaterial toxicity (fish early life stage, 0 HPF to 120 HPF). It includes information on the importance of acellular chorion removal, detailed chorion removal procedures, and a complete protocol for the toxicity assessment of nanomaterials using dechorionated zebrafish embryos. The focus of this document is on testing nanomaterial toxicity.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

ISO/TS 22082:2020 - Nanotechnologies: Assessment of nanomaterial toxicity using dechorionated zebrafish embryo specifies a rapid, standardized method for evaluating nanomaterial toxicity in early life‑stage zebrafish (0 HPF to 120 HPF). The technical specification describes why removal of the acellular chorion is important for nanomaterial exposure, gives detailed chorion‑removal procedures (enzymatic and mechanical), and presents a complete test protocol from culture and spawning to exposure, endpoint observation and data analysis.

Key topics and requirements

Scope and purpose: Rapid toxicity screening of engineered nanomaterials using dechorionated zebrafish embryos (0–120 HPF).

Chorion removal: Procedures for enzymatic dechorionation (e.g., pronase-based methods) and mechanical dechorionation (see Annex A). Advantages and limitations are discussed (enzymatic: high throughput and less mechanical damage; variability in enzyme activity can affect success).

Culture and spawning: Guidance on zebrafish strain, feeding, photoperiod, temperature, pH and spawning procedures (Annex B).

Test setup: Preparation of nanomaterial stock solutions, dispersion techniques, selection of testing concentrations and range‑finding tests.

Controls and validation: Use of a positive control (3,4‑dichloroaniline) and criteria for results validation (Annex C).

Exposure and endpoints: Water quality measurement, preparation and exposure of dechorionated embryos, observation of acute and sub‑acute endpoints up to 120 HPF.

Data analysis and reporting: Requirements for LC50/NOAEL determination, data handling and detailed test reporting (sections 7–8).

Applications and users

ISO/TS 22082:2020 is intended for:

Nanotoxicologists and ecotoxicologists conducting hazard screening of engineered nanomaterials.

Environmental safety and regulatory laboratories performing alternative in vivo assays.

Nanomaterial manufacturers and product stewardship teams assessing early‑stage toxicity.

Contract research organizations (CROs) seeking standardized, high‑throughput embryo assays. Practical benefits include faster screening, reduced use of mature vertebrates (animal welfare advantage), and improved ability to detect nanomaterial bioactivity that might be masked by the chorion.

Related standards

This Technical Specification references and aligns with existing nanotechnology and toxicity guidance, including:

ISO/TS 22082:2020 provides a practical, standardized approach for assessing nanomaterial toxicity using dechorionated zebrafish embryos, supporting reproducible screening and regulatory‑relevant reporting.

ISO/TS 22082:2020 PDF

Abstract

Похожие стандарты

Overview

Key topics and requirements

Applications and users

Related standards

Технические детали

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