ISO/TS 23459:2021 PDF

Name: ISO/TS 23459:2021 — Nanotechnologies — Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism
SKU: ISO/TS 23459:2021
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Nanotechnologies — Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism

Название (английский):

ISO/TS 23459:2021

Nanotechnologies — Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 26
Дата публикации:: 19 января 2021 г.
Издание:: ISO TS 23459 edition 1 version 1
ICS:: 07.120

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Описание (английский):

This document specifies measurement protocols and test conditions to determine alterations to protein secondary structure induced by their interaction with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy. This document does not apply to the characterization of conformational changes of disordered proteins.

Abstract

Похожие стандарты

Упомянутые в описании и другие стандарты ISO

Overview

ISO/TS 23459:2021 provides standardized measurement protocols to assess protein secondary structure changes that occur when proteins interact with nanomaterials using ultraviolet circular dichroism (UV‑CD) spectroscopy. The technical specification defines test conditions, sample preparation, instrumental settings and data analysis workflows to detect alterations in α‑helix, β‑sheet and other secondary structure elements. Note: this document does not apply to intrinsically disordered proteins.

Keywords: ISO/TS 23459:2021, UV‑CD, ultraviolet circular dichroism, protein secondary structure, nanomaterials, nanoparticle, protein–nanomaterial interactions.

Key topics and technical requirements

Scope & exclusions: Focused on secondary structure changes induced by nanomaterial–protein interactions; excludes characterization of disordered proteins.

Instrumentation: UV‑CD spectrometer with data range typically from 175 nm to 700 nm and temperature control.

Sample cells: Use highly transparent, non‑optically active quartz cells (rectangular or cylindrical) with path lengths 0.5 mm to 10.0 mm; thorough cleaning between runs is required.

Sample preparation:

Prepare protein stock solutions (recommended 1.0–5.0 mg/ml) in appropriate low‑absorption buffers.
Working concentrations for UV‑CD measurements typically 0.005–5.0 mg/ml, adjusted by path length and buffer.
Use low‑binding labware and deionized water; avoid optically active buffers.

Measurement procedures: Guidance on buffer selection, stability assessment of nanoparticle (NP) suspensions in protein solutions, and stepwise UV‑CD recording protocols.

Data processing: Calculation of molar ellipticity (mean residue ellipticity) and standardized data‑analysis methods to estimate secondary structure fractions.

Reporting: Required elements for a reproducible test report, plus informative annexes with typical spectra, buffer lists, unit conversions, concentration calculations and secondary‑structure estimation methods.

Applications and who should use it

Researchers studying nanomaterial–protein interactions, protein corona formation and nanoparticle bio‑reactivity.

Analytical and biophysical labs using CD spectroscopy for protein structural assessment in nanotoxicology, nanomedicine, formulation development and materials research.

Quality control and regulatory testing where standardized, reproducible methods for assessing protein conformational change are required.

Contract research organizations (CROs), pharmaceutical developers, academic groups and standards bodies.

Keywords: protein corona, nanotoxicology, CD spectroscopy, nanoparticle characterization, mean residue ellipticity.

ISO/TS 23459:2021 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and who should use it

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