ISO/TS 23973:2020 PDF
Liquid chromatography at critical conditions (LCCC) — Chemical heterogeneity of polyethylene oxides
Liquid chromatography at critical conditions (LCCC) — Chemical heterogeneity of polyethylene oxides
- Статус документа:
- Действующий
- Формат:
- Электронный (PDF)
- Количество страниц:
- 49
- Дата публикации:
- 17 августа 2020 г.
- Издание:
- ISO TS 23973 edition 1 version 1
- ICS:
- 71.040.50
This document establishes a valid method for separation of chemically heterogeneous polyethylene oxide (PEO) mixtures and for the determination the number and content of the chemically heterogeneous species in the overall sample. The method presented in this document serves as a technical guideline and enables laboratories to learn the principle of "critical chromatography" on a validated system. This method presented in this document with its stated system parameters is not applicable for other polymer classes, due to the diversity of the interactions between the polymer/mobile phase/stationary phase and the number of separation systems that are therefore available. The evaluation of the interlaboratory testing has shown that many error sources relate to the technique of liquid chromatography in general. Possible error sources are described in Annex A. Details on the evaluation of the interlaboratory testing are given in Annex B. Elugrams of the participants (excerpts) are given in Annex C. Investigations of the long-term stability of the test mixture are given in Annex D.
Abstract
Overview
ISO/TS 23973:2020 - "Liquid chromatography at critical conditions (LCCC) - Chemical heterogeneity of polyethylene oxides" is a technical specification from ISO that defines a validated LCCC method for separating chemically heterogeneous polyethylene oxide (PEO) mixtures and determining the number and relative content of chemically different species in a sample. The document provides a practical guideline for laboratories to learn and implement the principle of liquid chromatography at critical conditions (LCCC) on a validated system, including results from interlaboratory testing and annexes covering error sources and long‑term stability.
Key topics and technical requirements
- Principle: Identification of the critical solvent composition (csc) for the ethylene oxide repeat unit by adjusting eluent composition (example given: acetonitrile/water) until PEG standards elute independently of molar mass.
- Validated system components: requirements for eluent supply, pulse‑free pumps, injection systems (5 µl–25 µl), detectors (RI, ELSD, optional UV/IR), data acquisition and polymer‑compatible tubing.
- Columns and stationary phase: use of reversed‑phase C18, end‑capped, spherical porous packing (particle diameter
Технические детали
- Технический комитет
- ISO/TC 35/SC 16 - Chemical analysis
- SKU
- ISO/TS 23973:2020
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