ISO/TS 7833:2024 PDF

Name: ISO/TS 7833:2024 — Nanotechnologies — Extraction method of nanomaterials from lung tissue by proteinase K digestion
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Nanotechnologies — Extraction method of nanomaterials from lung tissue by proteinase K digestion

Название (английский):

ISO/TS 7833:2024

Nanotechnologies — Extraction method of nanomaterials from lung tissue by proteinase K digestion

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 14
Дата публикации:: 31 января 2024 г.
Издание:: ISO TS 7833 edition 1 version 1
ICS:: 07.120

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Описание (английский):

This document provides an extraction method using the proteinase K (PK) for nanomaterials deposited in the lung. This document specifies the advantages of the PK digestion method and examples of nanomaterials to which it can be applied. This document focuses on extracting nanomaterials from lung tissue and separating nanoparticles from their ionic counterparts. This method is potentially (or theoretically) applicable to any particles that are insoluble during the PK digestion process.

Abstract

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Overview

ISO/TS 7833:2024 - Nanotechnologies - Extraction method of nanomaterials from lung tissue by proteinase K digestion - specifies an enzymatic extraction method using proteinase K (PK) to recover nanomaterials deposited in lung tissue. The Technical Specification describes the advantages of PK digestion over aggressive chemical digestion (reduced particle dissolution and structural damage) and focuses on separating particulate nanomaterials from their ionic counterparts for subsequent instrumental analysis.

Key topics and technical requirements

Scope: Extraction of nanomaterials from lung tissue and separation of nanoparticles from dissolved ionic species; method is applicable to particles that remain insoluble during PK digestion.

Optimal PK system:

Recommended digestion buffer (tested formulation): 30 mM Tris‑HCl, 10 mM EDTA, 1% SDS, 5 mM CaCl2, pH 8.0.
Optimal PK activity reported in the document: 10 µg PK (≈ 0.2–0.3 U) under the described test conditions.
Incubation condition example: 56 °C ± 1 °C (heat block or water bath), typical digestion times in tests were 24 h.

Sample preparation & equipment:

Drying/weighting of lung tissue, slicing (~2 mm), homogenisation (micro ball mill), optional lyophiliser.
Vessels resistant to protein adsorption (1.5–50 ml tubes), Petri dishes for drying.
Centrifugation capable of ≥21 000 g (microcentrifuge/ultracentrifuge) to pellet nanoparticles; bath sonicator (≈400 W, 40 kHz) for dispersion.

Procedure outline:

Sampling and drying, homogenisation/powderisation, PK digestion of tissue, collection of nanoparticle pellet and preparation for instrumental analysis.

Methodological considerations:

Guidelines on separating particulate and ionic fractions, nanomaterials types applicable (e.g., carbon-based materials and certain metal oxides), and the impact of blood content or organ-specific variability.

Applications and users

Primary applications:

Lung burden analysis in inhalation toxicology and toxicokinetic studies.
Recovery and characterization of retained nanomaterials prior to ICP‑MS, sp-ICP‑MS, TEM, EDS, UV‑Vis or other instrumental analyses.

Who should use this standard:

Nanotoxicology laboratories, regulatory testing labs, contract research organisations (CROs), materials scientists, and researchers conducting inhalation or organ-distribution studies of engineered nanomaterials.

Related standards and guidance

ISO 80004‑1 (Nanotechnologies - Vocabulary)

ISO/TR 22019 (toxicokinetic considerations for nanomaterials)

OECD inhalation guidance (TG 412, TG 413) and ongoing toxicokinetic guidance initiatives (related to TG 417 discussions)

ISO/TS 7833:2024 provides a practical, validated PK-based protocol to extract particulate nanomaterials from lung tissue while minimising artefacts from chemical digestion - a key tool for accurate lung burden and toxicokinetic assessments.

ISO/TS 7833:2024 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards and guidance

Технические детали

SIST EN ISO 80004-1:2023

Nanotechnologies - Vocabulary - Part 1: Core vocabulary (ISO 80004-1:2023)

PD ISO/TR 22019:2019

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