PD CEN/TS 17883:2022 PDF

1   Scope This document specifies the crucial steps of a quantitative real-time polymerase chain reaction ( qPCR ) method to quantify the abundance of specific mRNA molecules extracted from Daphnia magna . The method allows the identification of molecular responses to exposures for potentially toxic substances through the analysis of the abundance of specific mRNA molecules. In this document, the central genes involved in reproductive and toxic responses are included.

NOTE The selection of genes can be adapted to specific exposure conditions, for example, exposure to known toxic substances, by adding genes known to respond to a specific insult.