Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Ovine DNA detection method
Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Ovine DNA detection method
What is ISO/TS 20224-2 about?
ISO/TS 20224-2 is the second-part of a multi-series of documents specifying a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep. The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration.
Who is ISO/TS 20224-2 for?
ISO/TS 20224-2 on molecular biomarker analysis is relevant to:
Molecular biomarker scientists
Другие стандарты PD