SIST EN ISO 10705-1:2001 PDF

Name: SIST EN ISO 10705-1:2001 — Water quality - Detection and enumeration of bacteriophages - Part 1: Enumeration of F-specific RNA bacteriophages (ISO 10705-1:1995)
SKU: SIST EN ISO 10705-1:2001
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Water quality - Detection and enumeration of bacteriophages - Part 1: Enumeration of F-specific RNA bacteriophages (ISO 10705-1:1995)

Название (английский):

SIST EN ISO 10705-1:2001

Water quality - Detection and enumeration of bacteriophages - Part 1: Enumeration of F-specific RNA bacteriophages (ISO 10705-1:1995)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 14
Дата публикации:: 1 декабря 2001 г.
ICS:: 07.100.20
Технический комитет:: KAV - Water quality

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Описание (английский):

Specifies a method for the detection and enumeration of F-specific ribonucleic acid bacteriophages. The sample is incubated with an appropriate host strain. Applicable to all kinds of water, sediments and sludges, even for shellfish extracts. The presence of F-specific RNA bacteriophages in a water sample indicates pollution by wastewater contaminated by human or animal faeces.

Abstract

Похожие стандарты

Стандарты, упомянутые в описании

Overview

EN ISO 10705-1:2001 (ISO 10705-1:1995) specifies a microbiological method for the detection and enumeration of F-specific RNA bacteriophages in water, sediments, sludges and shellfish extracts. The standard defines a host‑based plaque assay: samples are incubated with an appropriate host strain, plated on nutrient media and scored for visible plaques. Results are reported as plaque‑forming particles per unit volume and the presence of F‑specific RNA bacteriophages is interpreted as an indicator of wastewater faecal contamination from human or animal sources.

Key topics and technical requirements

Principle: incubation of sample with an F‑pilus host strain and enumeration of plaques on a bacterial lawn.

Applicability: all kinds of water, sediments, sludges and shellfish extracts; preconcentration recommended when phage numbers are low.

Confirmation: differential counts using RNase (typical inhibitory concentrations noted in the standard) to confirm F‑specific RNA phages.

Quality control: maintenance and QC of host strains (e.g., Salmonella typhimurium WG49), turbidity calibration, susceptibility testing and plate‑count performance checks.

Safety: management of host cultures (low‑pathogenic host strains) and prevention of cross‑contamination; work with high‑titre materials in appropriate containment (biosafety cabinet or dedicated area).

Reagents & media: use analytical‑grade chemicals and specified diluents/media; reference to ISO laboratory water and media preparation standards.

Results: expressed as concentration of plaque‑forming particles (cpfp or pfu) per unit volume.

Normative references: cross‑references to ISO 3696 (laboratory water), ISO 5667 series (sampling), ISO 6887, ISO 8199 and related microbiological standards.

Applications and users

Environmental and public‑health laboratories performing microbiological water quality monitoring.

Water and wastewater utilities assessing faecal pollution and treatment performance.

Shellfish monitoring programmes evaluating virus concentration/retention in shellfish.

Regulatory agencies and consultants requiring standardized methods for enteric virus surrogate monitoring.

Academic and applied researchers studying virus fate, transport and removal in environmental systems.

Related standards

ISO 10705‑2 (somatic coliphages) - companion part for other bacteriophage groups.

ISO 5667 series - guidance on sampling of water bodies.

ISO 3696, ISO 6887, ISO 8199 - laboratory water quality, dilution preparation and microbial enumeration guidance.

Keywords: water quality, F-specific RNA bacteriophages, detection and enumeration, ISO 10705-1, bacteriophage plaque assay, faecal contamination indicator, environmental microbiology.

SIST EN ISO 10705-1:2001 PDF

Abstract

Похожие стандарты

Overview

Key topics and technical requirements

Applications and users

Related standards

Технические детали

ISO 10705-1:1995

Water quality — Detection and enumeration of bacteriophages — Part 1: Enumeration of F-specific RNA bacteriop…

SIST EN ISO 3696:1998

Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

BS ISO 5667-21:2010

Water quality. Sampling - Guidance on sampling of drinking water distributed by tankers or means other than d…

ISO 6887-3:2017/Amd 1:2020

Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for mi…

SIST EN ISO 8199:2019

Water quality - General requirements and guidance for microbiological examinations by culture (ISO 8199:2018)

SIST EN ISO 10705-2:2001

Water quality - Detection and enumeration of bacteriophages - Part 2: Enumeration of somatic coliphages (ISO…