SIST EN ISO 11348-3:2009/A1:2019 PDF

Overview

EN ISO 11348-3:2008/A1:2018 amends the international method for assessing the inhibitory effect of water samples on the light emission of Vibrio fischeri - the luminescent bacteria test - specifically the variant that uses freeze-dried bacteria. Endorsed by CEN and prepared by ISO/TC 147 (Water quality), this amendment updates quality criteria within the existing Part 3 procedure (ISO 11348-3:2007) and is published in the three official CEN languages (EN/FR/DE).

Key topics and requirements

• Test principle: measurement of reduced bioluminescence of Vibrio fischeri as an indicator of sample toxicity (freeze-dried bacterial reagent format).
• Amendment focus: clarification of a precision/acceptance criterion in Clause 11 (third bullet) related to dilutions used to determine the LID‑value - the amendment specifies that, for the test sample dilutions determining the LID-value, the deviation from their mean (expressed in “percent points”) must not exceed 3 percentage points (see footnote c in Table 1).
• Quality control: tighter reproducibility/data acceptance rules for sample dilution results and reporting to ensure reliable interpretation of inhibitory effects.
• Standards governance: harmonized adoption across CEN member bodies; users should check national implementation dates and any national forewords.

Applications

• Environmental laboratories performing water quality testing and toxicity screening of surface water, effluents and treated wastewater.
• Regulatory agencies and compliance programs requiring standardized ecotoxicological assays.
• Contract testing and research facilities using luminescent bacteria tests for rapid, cost‑effective screening of chemical and biological contaminants.
• Manufacturers and suppliers of freeze‑dried Vibrio fischeri test kits who need to align product instructions and QC limits with the amended standard.
• Quality managers and method developers applying standard data acceptance criteria and reproducibility thresholds in routine monitoring.

Who should use this standard

• Environmental and public-health laboratories
• Water utilities and wastewater treatment operators
• Regulatory bodies and accreditation assessors
• Ecotoxicologists and environmental consultants
• Test kit producers and laboratory equipment vendors

Related standards

• This document is part of the ISO 11348 series on luminescent bacteria testing. For details on other parts and methods (fresh cultures, alternative procedures), consult the ISO website or national standards body.

Keywords: EN ISO 11348-3, ISO 11348-3:2007/Amd 1:2018, luminescent bacteria test, Vibrio fischeri, freeze-dried bacteria method, water quality testing, LID-value, ecotoxicology, CEN.